A competitive precision CRISPR method to identify the fitness effects of transcription factor binding sites.
Nat Biotechnol
; 41(2): 197-203, 2023 02.
Article
de En
| MEDLINE
| ID: mdl-36163549
ABSTRACT
Here we describe a competitive genome editing method that measures the effect of mutations on molecular functions, based on precision CRISPR editing using template libraries with either the original or altered sequence, and a sequence tag, enabling direct comparison between original and mutated cells. Using the example of the MYC oncogene, we identify important transcriptional targets and show that E-box mutations at MYC target gene promoters reduce cellular fitness.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Facteurs de transcription
/
Édition de gène
Type d'étude:
Prognostic_studies
Langue:
En
Journal:
Nat Biotechnol
Sujet du journal:
BIOTECNOLOGIA
Année:
2023
Type de document:
Article
Pays d'affiliation:
Finlande