Unadjuvanted intranasal spike vaccine elicits protective mucosal immunity against sarbecoviruses.
Science
; 378(6622): eabo2523, 2022 11 25.
Article
de En
| MEDLINE
| ID: mdl-36302057
ABSTRACT
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has highlighted the need for vaccines that not only prevent disease but also prevent transmission. Parenteral vaccines induce robust systemic immunity but poor immunity at the respiratory mucosa. We developed a vaccine strategy that we call "prime and spike," which leverages existing immunity generated by primary vaccination (prime) to elicit mucosal immune memory within the respiratory tract by using unadjuvanted intranasal spike boosters (spike). We show that prime and spike induces robust resident memory B and T cell responses, induces immunoglobulin A at the respiratory mucosa, boosts systemic immunity, and completely protects mice with partial immunity from lethal SARS-CoV-2 infection. Using divergent spike proteins, prime and spike enables the induction of cross-reactive immunity against sarbecoviruses.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Immunité muqueuse
/
Glycoprotéine de spicule des coronavirus
/
Vaccins contre la COVID-19
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SARS-CoV-2
/
COVID-19
/
Cellules B mémoire
/
Cellules T mémoire
/
Mémoire immunologique
Limites:
Animals
Langue:
En
Journal:
Science
Année:
2022
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique