Expression of transforming growth factor-ß1 and autophagy markers in the bladder of rats with neurogenic lower urinary tract injury.
Spinal Cord
; 61(2): 154-159, 2023 02.
Article
de En
| MEDLINE
| ID: mdl-36319684
ABSTRACT
OBJECTIVE:
This study was conducted to explore the expression of transforming growth factor-beta 1 (TGF-ß1) and its correlation with autophagy markers in the bladder of rats with neurogenic lower urinary tract dysfunction (NLUTD) post spinal cord injury (SCI). STUDYDESIGN:
A total of 36 male Wistar rats were randomly divided into the SCI group and control group. Rats in the SCI group were subjected to T10-T11 spinal cord transection. At day 1, 4, and 7, 6 rats were euthanized daily and the Basso, Beattie and Bresnahan score (BBB score), post-void residual (PVR), urinary bladder function score (UBFS) and bladder weight were assessed. The expression TGF-ß1 and autophagy markers were evaluated by immunofluorescence staining, Western bolt, and qRT-PCR.SETTING:
A total of 36 male Wistar rats were randomly divided into the SCI group and control group, with three time points in each group.PARTICIPANTS:
Not applicable.RESULTS:
SCI modeling impaired the motor function of the hind limbs and the bladder function of rats. NLUTD muscle exhibited a punctated immunostaining pattern for LC3, suggesting the accumulation of autophagosomes. Our results further indicated that compared with the control group, the expression levels of TGF-ß1 and LC3 were increased, while the level of P62 was decreased after SCI modeling.CONCLUSION:
TGF-ß1 was significantly increased in SCI rats with NLUTD and was correlated with autophagy markers LC3 and p62.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Traumatismes de la moelle épinière
/
Maladies urologiques
/
Vessie neurologique
Type d'étude:
Prognostic_studies
Limites:
Animals
Langue:
En
Journal:
Spinal Cord
Sujet du journal:
NEUROLOGIA
Année:
2023
Type de document:
Article
Pays d'affiliation:
Chine