Split-Type Photoelectrochemical/Visual Sensing Platform Based on SnO2/MgIn2S4/Zn0.1Cd0.9S Composites and Au@Fe3O4 Nanoparticles for Ultrasensitive Detection of Neuron Specific Enolase.
Anal Chem
; 94(45): 15873-15878, 2022 11 15.
Article
de En
| MEDLINE
| ID: mdl-36322926
ABSTRACT
Herein, a novel dual mode detection system of split-type photoelectrochemical (PEC) and visual immunoassay was developed to detect neuron specific enolase (NSE), which achieved simultaneous and reliable NSE detection due to the completely different signal readouts and transduction mechanism. Specifically, specific reactions of antigens and antibodies were performed in 96-microwell plates. Gold nanoparticle (Au NP)-loaded Fe3O4 (Au@Fe3O4) NPs were used as secondary antibody markers and signal regulators, which could produce a blue-colored solution in the presence of 3,3',5,5'-tetramethylbenzidine (TMB) and H2O2 because of its peroxidase-like activity. Therefore, the visual detection of NSE was realized, making the results more intuitive. Meanwhile, the above biological process could also be used as part of the split-type PEC sensing platform. Oxidized TMB and Fe3+ were consumptive agents of the electron donor, which both realized the double quenching of PEC signal generated by the SnO2/MgIn2S4/Zn0.1Cd0.9S composites. Owing to the waterfall band structure, SnO2/MgIn2S4/Zn0.1Cd0.9S composites partially absorb visible light and effectively inhibit the electron-hole recombination, thereby providing significantly enhanced and stable initial signal. On the basis of the multiple signal amplification strategy and the split-type mode, NSE could be sensitively detected with a low detection limit of 14.0 fg·mL-1 (S/N = 3) and a wide linear range from 50.0 fg·mL-1 to 50.0 ng·mL-1.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Techniques de biocapteur
/
Nanoparticules métalliques
Type d'étude:
Diagnostic_studies
Langue:
En
Journal:
Anal Chem
Année:
2022
Type de document:
Article