Automated assembly scaffolding using RagTag elevates a new tomato system for high-throughput genome editing.
Genome Biol
; 23(1): 258, 2022 12 15.
Article
de En
| MEDLINE
| ID: mdl-36522651
ABSTRACT
Advancing crop genomics requires efficient genetic systems enabled by high-quality personalized genome assemblies. Here, we introduce RagTag, a toolset for automating assembly scaffolding and patching, and we establish chromosome-scale reference genomes for the widely used tomato genotype M82 along with Sweet-100, a new rapid-cycling genotype that we developed to accelerate functional genomics and genome editing in tomato. This work outlines strategies to rapidly expand genetic systems and genomic resources in other plant species.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Solanum lycopersicum
Langue:
En
Journal:
Genome Biol
Sujet du journal:
BIOLOGIA MOLECULAR
/
GENETICA
Année:
2022
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique