Generation of orthotopic patient-derived xenograft models for pancreatic cancer using tumor slices.
STAR Protoc
; 3(4): 101899, 2022 12 16.
Article
de En
| MEDLINE
| ID: mdl-36595938
ABSTRACT
Orthotopic patient-derived xenograft models recapitulate the genomic complexity of the original tumor and some aspects of local microenvironment, useful for rapid development and validation of personalized treatment strategies. Here, we precisely describe a protocol for generating tumor slices from human or murine-derived pancreatic cancer. They are then implanted directly into the murine pancreas, monitored using ultrasound, with a 90% success rate. This assay creates a clinically relevant in vivo model facilitating personalized treatment development.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Tumeurs du pancréas
Type d'étude:
Guideline
Limites:
Animals
/
Humans
Langue:
En
Journal:
STAR Protoc
Année:
2022
Type de document:
Article