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[Mechanism of Suanzaoren Decoction in improving insomnia rats by integrating metabolomics and intestinal flora analysis].
DU, He; Liu, Jia-Xing; Yan, Yan; DU, Chen-Hui.
Affiliation
  • DU H; School of Traditional Chinese Materia Medica, Shanxi University of Chinese Medicine Taiyuan 030619,China.
  • Liu JX; Modern Research Center for Traditional Chinese Medicine, Shanxi University Taiyuan 030006, China.
  • Yan Y; Modern Research Center for Traditional Chinese Medicine, Shanxi University Taiyuan 030006, China.
  • DU CH; School of Traditional Chinese Materia Medica, Shanxi University of Chinese Medicine Taiyuan 030619,China.
Zhongguo Zhong Yao Za Zhi ; 47(24): 6741-6752, 2022 Dec.
Article de Zh | MEDLINE | ID: mdl-36604924
ABSTRACT
To explore the mechanism of Suanzaoren Decoction(SZRD) in improving the insomnia rat model induced by DL-4-chlorophenylalanine(PCPA). The insomnia model was established by single intraperitoneal injection with PCPA(400 mg·kg~(-1)), UPLC-Q-TOF-MS/MS was used to analyze the profile of metabolites in rat hippocampus samples, combined with multivariate statistical analysis and screening of differential metabolites, and related metabolic pathways were constructed with MetaboAnalyst 5.0. The high-throughput sequencing of V3-V4 regions of 16 S rRNA gene was used to predict the structure and relative abundance of intestinal flora by LEfSe, OPLS-DA and PICRUSt2. A total of 22 differential hippocampus metabolites were identified by metabolomics analysis, including amino acids, fatty acids, nucleosides, organic acids, vitamins, and others. Pathway analysis showed that alanine, aspartate and glutamic metabolism, D-glutamine and D-glutamate metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, arginine biosynthesis were the main pathways. 16 S rRNA gene sequencing showed that Ruminococcus and Eubacterium were the differences between SZRD group and model group. Ruminococcus might be the sign of SZRD improving PCPA insomnia on analysis of PICRUSt2 and LEfSe. Furthermore Spearman correlation analysis showed that the differential metabolites 2-oxo-4-methylthiobutyric acid and palmitic acid intervened by SZRD were significantly positively correlated with the differential flora. In conclusion, SZRD indirectly improves insomnia by affecting metabolic pathways such as amino acids metabolic pathways and regulating the structure of flora. The results of this study provide a new mechanism and new idea for elucidating the mechanism of classic famous prescription SZRD in improving insomnia from the perspective of intestinal flora.
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Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Médicaments issus de plantes chinoises / Microbiome gastro-intestinal / Troubles de l'endormissement et du maintien du sommeil Type d'étude: Prognostic_studies Limites: Animals Langue: Zh Journal: Zhongguo Zhong Yao Za Zhi Sujet du journal: FARMACOLOGIA / TERAPIAS COMPLEMENTARES Année: 2022 Type de document: Article Pays d'affiliation: Chine

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Médicaments issus de plantes chinoises / Microbiome gastro-intestinal / Troubles de l'endormissement et du maintien du sommeil Type d'étude: Prognostic_studies Limites: Animals Langue: Zh Journal: Zhongguo Zhong Yao Za Zhi Sujet du journal: FARMACOLOGIA / TERAPIAS COMPLEMENTARES Année: 2022 Type de document: Article Pays d'affiliation: Chine