Biomimetic nanochannels for molybdate transport: application to sensitive electrochemical immunoassay for HER2.
Mikrochim Acta
; 190(2): 53, 2023 01 14.
Article
de En
| MEDLINE
| ID: mdl-36640214
A nanochannel-based electrochemical immunoassay was developed for the detection of human epidermal growth factor receptor 2 (HER2), with molybdate as the reporter to explore the interaction occurring into the nanochannels. The presence of target increased steric hindrance of the antibody-functionalized nanochannels, thereby hindering the transport of molybdate. And the reporter could be monitored by working electrode modified with hydroxyapatite nanoparticles, based on the formation of the redox-active molybdophosphate. As a result, peak current obtained at ca. - 0.28 V in square wave voltammograms could be applied to quantitative determination of HER2. The electrochemical signal increased linearly with the logarithm of the concentration of HER2 in a broad dynamic range of 0.1 pgâmL-1 to 10 ngâmL-1 with a detection limit of 0.05 pgâmL-1. The reliability of this immunoassay was validated by a recovery range of 99.5% to 111.7% for the detection of three different levels of HER2 in human serum samples. Integrating with multiple bionanochannels, this immunoassay is expected to provide a versatile approach for quantitative detection of various biomarkers in related disease diagnosis and therapy.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Biomimétique
/
Molybdène
Type d'étude:
Diagnostic_studies
Limites:
Humans
Langue:
En
Journal:
Mikrochim Acta
Année:
2023
Type de document:
Article
Pays d'affiliation:
Chine
Pays de publication:
Autriche