Protocol for genome-scale in vivo CRISPR screening to study protection of beta cells under autoimmunity in a type 1 diabetes mouse model.

Li, Jian; Lee, Yu-Chi; Iessi, Isabela L; Wu, Chialing; Yi, Peng; Cai, Erica P

Protocol for genome-scale in vivo CRISPR screening to study protection of beta cells under autoimmunity in a type 1 diabetes mouse model.

Li, Jian; Lee, Yu-Chi; Iessi, Isabela L; Wu, Chialing; Yi, Peng; Cai, Erica P.

Affiliation

Li J; Section for Islet Cell and Regenerative Biology, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA.
Lee YC; Section for Islet Cell and Regenerative Biology, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA.
Iessi IL; Lilly Diabetes Center of Excellence, Indiana Biosciences Research Institute, Indianapolis, IN 46202, USA.
Wu C; Lilly Diabetes Center of Excellence, Indiana Biosciences Research Institute, Indianapolis, IN 46202, USA.
Yi P; Section for Islet Cell and Regenerative Biology, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA. Electronic address: peng.yi@joslin.harvard.edu.
Cai EP; Lilly Diabetes Center of Excellence, Indiana Biosciences Research Institute, Indianapolis, IN 46202, USA; Center for Diabetes and Metabolic Diseases, Indiana University School of Medicine, Indianapolis, IN 46202, USA. Electronic address: ecai@indianabiosciences.org.

STAR Protoc ; 4(2): 102155, 2023 Mar 13.

Article de En | MEDLINE | ID: mdl-36917606

Mots clés

CRISPR; Immunology; Metabolism; Sequencing

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Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Type d'étude: Diagnostic_studies / Prognostic_studies / Screening_studies Langue: En Journal: STAR Protoc Année: 2023 Type de document: Article Pays d'affiliation: États-Unis d'Amérique Pays de publication: États-Unis d'Amérique

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