X-ray inhibits FUT4-mediated proliferation in A549 cells by downregulating SP1 expression.
J Pak Med Assoc
; 73(3): 494-499, 2023 Mar.
Article
de En
| MEDLINE
| ID: mdl-36932748
ABSTRACT
Objective:
To identify the mechanism of down-regulation of Lewis Y antigen caused by X-ray irradiation.METHODS:
The present original research study was conducted at Zhejiang University City College, Hangzhou, Republic of China, from 2020 to 2022. Western blotting, Co-immunoprecipitation (CO-IP), electrophoretic mobility shift assay and Cell Counting Kit-8 (CCK8) were performed to confirm the effect of X-ray irradiation on A549 cell proliferation and its mechanism. Data was analysed using Statistical Package for Social Sciences (SPSS) 11.5.RESULTS:
The expressions of fucosyltransferase IV and Lewis Y were decreased after X-ray irradiation, thus inhibiting the proliferation of A549 lung cancer cells. Deoxyribonucleic acid damage caused by the irradiation caused higher level of poly- adenosinediphosphate-ribosylated Specific Protein 1(SP1), and translocation of SP1 from the nucleus, decreasing the expression of fucosyltransferase IV and Lewis Y.Conclusion:
There was a significant role of glycosylation in radiation therapy for lung cancer.Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Rayons X
/
Facteur de transcription Sp1
/
Fucosyltransferases
/
/
Tumeurs du poumon
Type d'étude:
Prognostic_studies
Limites:
Humans
Langue:
En
Journal:
J Pak Med Assoc
Année:
2023
Type de document:
Article
Pays d'affiliation:
Chine
Pays de publication:
PAKISTAN
/
PAQUISTÃO
/
PK