Isolation of primary human liver cells from normal and nonalcoholic steatohepatitis livers.
STAR Protoc
; 4(3): 102391, 2023 Sep 15.
Article
de En
| MEDLINE
| ID: mdl-37405925
ABSTRACT
Here, we present a protocol for isolating human hepatocytes and neural progenitor cells from normal and nonalcoholic steatohepatitis livers. We describe steps for perfusion for scaled-up liver cell isolation and optimization of chemical digestion to achieve maximal yield and cell viability. We then detail a liver cell cryopreservation and potential applications, such as the use of human liver cells as a tool to link experimental and translational research.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Stéatose hépatique non alcoolique
Limites:
Humans
Langue:
En
Journal:
STAR Protoc
Année:
2023
Type de document:
Article