The identification of diurnal variations on circulating immune cells by finger prick blood sampling in small sample sizes: a pilot study.
Lab Med
; 55(2): 220-226, 2024 Mar 07.
Article
de En
| MEDLINE
| ID: mdl-37478465
ABSTRACT
OBJECTIVE:
There are well-described impacts of biological rhythms on human physiology. With the increasing push for routine blood tests for preventative medical care and clinical and physiological research, optimizing effectiveness is paramount. This study aimed to determine whether it is feasible to assess diurnal variations of peripheral lymphocyte prevalence using finger prick blood in a small sample size.METHODS:
Using polychromatic flow cytometry, the prevalence of lymphocytes was assessed using 25 µL fingertip blood samples at 8 AM and 5 PM from 8 participants.RESULTS:
TH cells and B cells showed significantly higher percentages in the 5 PM samples, whereas NK cells demonstrated a significantly higher morning percentage. T cells, leukocytes, and cytotoxic T cells showed no significant changes.CONCLUSION:
The detection of diurnal variations demonstrates that small blood volumes can be used to detect lymphocyte variations. The lower blood volume required provides a new testing method for clinical and research settings.Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Cellules tueuses naturelles
/
Lymphocytes T
Type d'étude:
Diagnostic_studies
/
Risk_factors_studies
Limites:
Humans
Langue:
En
Journal:
Lab Med
Année:
2024
Type de document:
Article
Pays d'affiliation:
Australie