Your browser doesn't support javascript.
loading
A single-cell transcriptional landscape of immune cells shows disease-specific changes of T cell and macrophage populations in human achalasia.
Liu, Zu-Qiang; Dai, Hao; Yao, Lu; Chen, Wei-Feng; Wang, Yun; Ma, Li-Yun; Li, Xiao-Qing; Lin, Sheng-Li; He, Meng-Jiang; Gao, Ping-Ting; Liu, Xin-Yang; Xu, Jia-Xin; Xu, Xiao-Yue; Wang, Ke-Hao; Wang, Li; Chen, Luonan; Zhou, Ping-Hong; Li, Quan-Lin.
Affiliation
  • Liu ZQ; Endoscopy Center and Endoscopy Research Institute, Zhongshan Hospital, Fudan University, Shanghai, China.
  • Dai H; Shanghai Collaborative Innovation Center of Endoscopy, Shanghai, China.
  • Yao L; Key Laboratory of Systems Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, China.
  • Chen WF; Endoscopy Center and Endoscopy Research Institute, Zhongshan Hospital, Fudan University, Shanghai, China.
  • Wang Y; Shanghai Collaborative Innovation Center of Endoscopy, Shanghai, China.
  • Ma LY; Endoscopy Center and Endoscopy Research Institute, Zhongshan Hospital, Fudan University, Shanghai, China.
  • Li XQ; Shanghai Collaborative Innovation Center of Endoscopy, Shanghai, China.
  • Lin SL; Endoscopy Center and Endoscopy Research Institute, Zhongshan Hospital, Fudan University, Shanghai, China.
  • He MJ; Shanghai Collaborative Innovation Center of Endoscopy, Shanghai, China.
  • Gao PT; Endoscopy Center and Endoscopy Research Institute, Zhongshan Hospital, Fudan University, Shanghai, China.
  • Liu XY; Shanghai Collaborative Innovation Center of Endoscopy, Shanghai, China.
  • Xu JX; Endoscopy Center and Endoscopy Research Institute, Zhongshan Hospital, Fudan University, Shanghai, China.
  • Xu XY; Shanghai Collaborative Innovation Center of Endoscopy, Shanghai, China.
  • Wang KH; Endoscopy Center and Endoscopy Research Institute, Zhongshan Hospital, Fudan University, Shanghai, China.
  • Wang L; Shanghai Collaborative Innovation Center of Endoscopy, Shanghai, China.
  • Chen L; Endoscopy Center and Endoscopy Research Institute, Zhongshan Hospital, Fudan University, Shanghai, China.
  • Zhou PH; Shanghai Collaborative Innovation Center of Endoscopy, Shanghai, China.
  • Li QL; Endoscopy Center and Endoscopy Research Institute, Zhongshan Hospital, Fudan University, Shanghai, China.
Nat Commun ; 14(1): 4685, 2023 08 04.
Article de En | MEDLINE | ID: mdl-37542039
ABSTRACT
Achalasia is a rare motility disorder of the esophagus caused by the gradual degeneration of myenteric neurons. Immune-mediated ganglionitis has been proposed to underlie the loss of myenteric neurons. Here, we measure the immune cell transcriptional profile of paired lower esophageal sphincter (LES) tissue and blood samples in achalasia and controls using single-cell RNA sequencing (scRNA-seq). In achalasia, we identify a pattern of expanded immune cells and a specific transcriptional phenotype, especially in LES tissue. We show C1QC+ macrophages and tissue-resident memory T cells (TRM), especially ZNF683+ CD8+ TRM and XCL1+ CD4+ TRM, are significantly expanded and localized surrounding the myenteric plexus in the LES tissue of achalasia. C1QC+ macrophages are transcriptionally similar to microglia of the central nervous system and have a neurodegenerative dysfunctional phenotype in achalasia. TRM also expresses transcripts of dysregulated immune responses in achalasia. Moreover, inflammation increases with disease progression since immune cells are more activated in type I compared with type II achalasia. Thus, we profile the immune cell transcriptional landscape and identify C1QC+ macrophages and TRM as disease-associated immune cell subsets in achalasia.
Sujet(s)

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Achalasie oesophagienne Limites: Humans Langue: En Journal: Nat Commun Sujet du journal: BIOLOGIA / CIENCIA Année: 2023 Type de document: Article Pays d'affiliation: Chine

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Achalasie oesophagienne Limites: Humans Langue: En Journal: Nat Commun Sujet du journal: BIOLOGIA / CIENCIA Année: 2023 Type de document: Article Pays d'affiliation: Chine
...