Single-cell analysis of human MAIT cell transcriptional, functional and clonal diversity.
Nat Immunol
; 24(9): 1565-1578, 2023 09.
Article
de En
| MEDLINE
| ID: mdl-37580605
ABSTRACT
Mucosal-associated invariant T (MAIT) cells are innate-like T cells that recognize microbial metabolites through a semi-invariant T cell receptor (TCR). Major questions remain regarding the extent of human MAIT cell functional and clonal diversity. To address these, we analyzed the single-cell transcriptome and TCR repertoire of blood and liver MAIT cells and developed functional RNA-sequencing, a method to integrate function and TCR clonotype at single-cell resolution. MAIT cell clonal diversity was comparable to conventional memory T cells, with private TCR repertoires shared across matched tissues. Baseline functional diversity was low and largely related to tissue site. MAIT cells showed stimulus-specific transcriptional responses in vitro, with cells positioned along gradients of activation. Clonal identity influenced resting and activated transcriptional profiles but intriguingly was not associated with the capacity to produce IL-17. Overall, MAIT cells show phenotypic and functional diversity according to tissue localization, stimulation environment and clonotype.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Cellules T invariantes associées aux muqueuses
Limites:
Humans
Langue:
En
Journal:
Nat Immunol
Sujet du journal:
ALERGIA E IMUNOLOGIA
Année:
2023
Type de document:
Article
Pays d'affiliation:
Royaume-Uni