Molecular detection of Toxoplasma gondii, Neospora caninum and Sarcocystis spp in tissues of Sus scrofa slaughtered in southern Brazil.
Rev Bras Parasitol Vet
; 32(3): e004623, 2023.
Article
de En
| MEDLINE
| ID: mdl-37585953
ABSTRACT
The aim of this study was to determine the presence of deoxyribonucleic acid (DNA) from Toxoplasma gondii, Sarcocystis spp. and Neospora caninum, in tissues of wild boars slaughtered in southern Brazil. A total of 156 samples were collected from different organs of 25 wild boars, and DNA from at least one of the protozoa investigated was detected in 79 samples. To differentiate between infectious agents, restriction fragment length polymorphism was performed using the restriction enzymes DdeI and HpaII. For N. caninum, conventional PCR was performed with specific primers. The DNA of at least one of the studied pathogens was detected in each animal 26.58% for T. gondii, 68.36% for Sarcocystis spp. and 5.06% for N. caninum. Coinfection between T. gondii and Sarcocystis spp. occurred in 14 animals, between T. gondii and N. caninum in only one male animal, between Sarcocystis spp. and N. caninum in a female, while co-infection with the three agents was equally observed in only one male animal. Considering the high frequency of detection and its zoonotic risk, especially T. gondii, it appears that wild boars can be potential sources of transmission of infectious agents and the adoption of monitoring measures in these populations should be prioritized.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Toxoplasmose animale
/
Sarcocystose
/
Coccidiose
/
Sus scrofa
Type d'étude:
Diagnostic_studies
Limites:
Animals
Pays/Région comme sujet:
America do sul
/
Brasil
Langue:
En
Journal:
Rev Bras Parasitol Vet
Sujet du journal:
MEDICINA VETERINARIA
/
PARASITOLOGIA
Année:
2023
Type de document:
Article
Pays d'affiliation:
Brésil