High-throughput differential scanning fluorimetry (DSF) and cellular thermal shift assays (CETSA): Shifting from manual to automated screening.
SLAS Technol
; 28(6): 411-415, 2023 12.
Article
de En
| MEDLINE
| ID: mdl-37598756
ABSTRACT
Biophysical affinity screening is increasingly being adopted as a high-throughput hit finding technique in drug discovery. Automation is highly beneficial to high-throughput screening (HTS) since a large number of compounds need to be reproducibly tested against a biological target. Herein, we describe how we have automated two biophysical affinity screening methods that rely on a thermal shift in protein melting temperature upon small molecule binding differential scanning fluorimetry (DSF) and the cellular thermal shift assay (CETSA).
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Découverte de médicament
/
Tests de criblage à haut débit
Type d'étude:
Diagnostic_studies
/
Screening_studies
Langue:
En
Journal:
SLAS Technol
Année:
2023
Type de document:
Article