Methionine Restriction Increases Exosome Production and Secretion in Breast Cancer Cells.
Cancer Genomics Proteomics
; 20(5): 412-416, 2023.
Article
de En
| MEDLINE
| ID: mdl-37643781
ABSTRACT
BACKGROUND/AIM:
Methionine addiction is the elevated requirement for exogenous methionine for growth and survival of cancer cells, termed the Hoffman effect. Methionine-addicted cancer cells synthesize normal or excess amounts of methionine but still need an external source of methionine. Methionine restriction (MR) by either a methionine-free medium or in vivo by a low-methionine diet or by methioninase, selectively arrests cancer cells in the late S/G2 cell cycle phase, but not normal cells. The present study focuses on the comparison of production and secretion of exosomes by cancer cells under MR and normal conditions. MATERIALS ANDMETHODS:
MDA-MB-231 cells (triple-negative breast cancer), containing exosomes labeled with CD63-GFP (CD63-GFP exosomes), were visualized by fluorescence microscopy. MDA-MB-231 cells expressing exosome-specific CD63-GFP were cultured in methionine-containing (MET+) or in methionine-free (MET-) DMEM conditions. Exosomes were isolated from conditioned medium of cultured MDA-MD-231 cells by ultracentrifugation and characterized by nanoparticle tracking analysis (NTA) and Western blotting.RESULTS:
When MDA-MB-231-CD63-GFP cells were cultured under MR conditions, they arrested their growth and CD63-GFP-expressing exosomes were strongly increased in the cells. MR resulted in approximately a 2-fold increase in exosome production and secretion per cell, even though cell growth was arrested. Methionine restriction thus resulted in elevated exosome production and secretion per surviving cell.CONCLUSION:
Exosome production and secretion in the cancer cells increased under MR, suggesting a relation between MR and exosome production and secretion.Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Tumeurs du sein
/
Exosomes
Limites:
Female
/
Humans
Langue:
En
Journal:
Cancer Genomics Proteomics
Sujet du journal:
BIOQUIMICA
/
GENETICA MEDICA
/
NEOPLASIAS
Année:
2023
Type de document:
Article