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Production of a p65fl/fl/LysMCre mouse model with dysfunctional NF-κB signaling in bone marrow-derived macrophages.
Korkaya, Ahmet K; Fischer, Jeffrey; Peppers, Anthony; Crosson, Sean M; Rayamajhi, Manira; Miao, Edward A; Baldwin, Albert S; Bradford, Jennifer W.
Affiliation
  • Korkaya AK; Department of Biological Sciences, Augusta University, Augusta, Georgia, USA.
  • Fischer J; Department of Biological Sciences, Augusta University, Augusta, Georgia, USA.
  • Peppers A; Department of Biological Sciences, Augusta University, Augusta, Georgia, USA.
  • Crosson SM; Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
  • Rayamajhi M; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
  • Miao EA; Department of Integrative Immunobiology, Duke University, Durham, North Carolina, USA.
  • Baldwin AS; Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
  • Bradford JW; Department of Biological Sciences, Augusta University, Augusta, Georgia, USA.
Innate Immun ; 29(8): 171-185, 2023 Nov.
Article de En | MEDLINE | ID: mdl-37828842
ABSTRACT
Here, we describe the production and characterization of a novel p65fl/fl/LysMCre mouse model, which lacks canonical nuclear factor-kappaB member RelA/p65 (indicated as p65 hereafter) in bone marrow-derived macrophages. Cultured bone marrow-derived macrophages that lack p65 protein reveal NF-κB signaling deficiencies, a reduction in phagocytic ability, and reduced ability to produce nitrites. Despite abnormal bone marrow-derived macrophage function, p65fl/fl/LysMCre mice do not exhibit differences in naïve systemic immune profiles or colony forming units and time to death following Salmonella infection as compared to controls. Additionally, p65fl/fl/LysMCre mice, especially females, display splenomegaly, but no other obvious physical or behavioral differences as compared to control animals. As bone marrow-derived macrophages from this transgenic model are almost completely devoid of canonical nuclear factor-kappaB pathway member p65, this model has the potential for being very useful in investigating bone marrow-derived macrophage NF-kappaB signaling in diverse biological and biomedical studies.
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Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Transduction du signal / Facteur de transcription NF-kappa B Limites: Animals Langue: En Journal: Innate Immun Sujet du journal: ALERGIA E IMUNOLOGIA / BACTERIOLOGIA Année: 2023 Type de document: Article Pays d'affiliation: États-Unis d'Amérique

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Transduction du signal / Facteur de transcription NF-kappa B Limites: Animals Langue: En Journal: Innate Immun Sujet du journal: ALERGIA E IMUNOLOGIA / BACTERIOLOGIA Année: 2023 Type de document: Article Pays d'affiliation: États-Unis d'Amérique