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The slan antigen identifies the prototypical non-classical CD16+-monocytes in human blood.
Tamassia, Nicola; Bianchetto-Aguilera, Francisco; Gasperini, Sara; Grimaldi, Alessio; Montaldo, Claudia; Calzetti, Federica; Gardiman, Elisa; Signoretto, Ilaria; Castellucci, Monica; Barnaba, Vincenzo; Tripodi, Marco; Cassatella, Marco Antonio.
Affiliation
  • Tamassia N; Department of Medicine, Section of General Pathology, University of Verona, Verona, Italy.
  • Bianchetto-Aguilera F; Department of Medicine, Section of General Pathology, University of Verona, Verona, Italy.
  • Gasperini S; Department of Medicine, Section of General Pathology, University of Verona, Verona, Italy.
  • Grimaldi A; Department of Internal Clinical Sciences, Anesthesiology and Cardiovascular Sciences, Sapienza University of Rome, Rome, Italy.
  • Montaldo C; Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy.
  • Calzetti F; National Institute for Infectious Diseases L. Spallanzani, IRCCS, Rome, Italy.
  • Gardiman E; Department of Medicine, Section of General Pathology, University of Verona, Verona, Italy.
  • Signoretto I; Department of Medicine, Section of General Pathology, University of Verona, Verona, Italy.
  • Castellucci M; Department of Medicine, Section of General Pathology, University of Verona, Verona, Italy.
  • Barnaba V; Centro Piattaforme Tecnologiche, University of Verona, Verona, Italy.
  • Tripodi M; Department of Internal Clinical Sciences, Anesthesiology and Cardiovascular Sciences, Sapienza University of Rome, Rome, Italy.
  • Cassatella MA; Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy.
Front Immunol ; 14: 1287656, 2023.
Article de En | MEDLINE | ID: mdl-37965335
ABSTRACT

Introduction:

Peripheral monocytes in humans are conventionally divided into classical (CL, CD14++CD16-), intermediate (INT, CD14++CD16+) and non-classical (NC, CD14dim/-CD16++) cells, based on their expression levels of CD14 and CD16. A major fraction of the NC-monocytes has been shown to express the 6-sulfo LacNAc (slan) antigen, but whether these slan+/NC-monocytes represent the prototypical non-classical monocytes or whether they are simply a sub-fraction with identical features as the remainder of NC monocytes is still unclear.

Methods:

We analyzed transcriptome (by bulk and single cell RNA-seq), proteome, cell surface markers and production of discrete cytokines by peripheral slan+/NC- and slan-/NC-monocytes, in comparison to total NC-, CL- and INT- monocytes.

Results:

By bulk RNA-seq and proteomic analysis, we found that slan+/NC-monocytes express higher levels of genes and proteins specific of NC-monocytes than slan-/NC-monocytes do. Unsupervised clustering of scRNA-seq data generated one cluster of NC- and one of INT-monocytes, where all slan+/NC-monocytes were allocated to the NC-monocyte cluster, while slan-/NC-monocytes were found, in part (13.4%), within the INT-monocyte cluster. In addition, total NC- and slan-/NC-monocytes, but not slan+/NC-monocytes, were found by both bulk RNA-seq and scRNA-seq to contain a small percentage of natural killer cells.

Conclusion:

In addition to comparatively characterize total NC-, slan-/NC- and slan+/NC-monocyte transcriptomes and proteomes, our data prove that slan+/NC-, but not slan-/NC-, monocytes are more representative of prototypical NC-monocytes.
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Mots clés

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Monocytes / Protéomique Limites: Humans Langue: En Journal: Front Immunol Année: 2023 Type de document: Article Pays d'affiliation: Italie

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Monocytes / Protéomique Limites: Humans Langue: En Journal: Front Immunol Année: 2023 Type de document: Article Pays d'affiliation: Italie