High-performance enrichment-based genome sequencing to support the investigation of hepatitis A virus outbreaks.
Microbiol Spectr
; 12(1): e0283423, 2024 Jan 11.
Article
de En
| MEDLINE
| ID: mdl-38018979
ABSTRACT
IMPORTANCE This proof-of-concept study introduces a hybrid capture oligo panel for whole-genome sequencing of all six human pathogenic hepatitis A virus (HAV) subgenotypes, exhibiting a higher sensitivity than some conventional genotyping assays. The ability of hybrid capture to enrich multiple targets allows for a single, streamlined workflow, thus facilitating the potential harmonization of molecular surveillance of HAV with other enteric viruses. Even challenging sample matrices can be accommodated, making them suitable for broad implementation in clinical and public health laboratories. This innovative approach has significant implications for enhancing multijurisdictional outbreak investigations as well as our understanding of the global diversity and transmission dynamics of HAV.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Virus de l'hépatite A
/
Hépatite A
Limites:
Humans
Langue:
En
Journal:
Microbiol Spectr
Année:
2024
Type de document:
Article
Pays d'affiliation:
Australie