Structural mechanism of outer kinetochore Dam1-Ndc80 complex assembly on microtubules.
Science
; 382(6675): 1184-1190, 2023 12 08.
Article
de En
| MEDLINE
| ID: mdl-38060647
ABSTRACT
Kinetochores couple chromosomes to the mitotic spindle to segregate the genome during cell division. An error correction mechanism drives the turnover of kinetochore-microtubule attachments until biorientation is achieved. The structural basis for how kinetochore-mediated chromosome segregation is accomplished and regulated remains an outstanding question. In this work, we describe the cryo-electron microscopy structure of the budding yeast outer kinetochore Ndc80 and Dam1 ring complexes assembled onto microtubules. Complex assembly occurs through multiple interfaces, and a staple within Dam1 aids ring assembly. Perturbation of key interfaces suppresses yeast viability. Force-rupture assays indicated that this is a consequence of impaired kinetochore-microtubule attachment. The presence of error correction phosphorylation sites at Ndc80-Dam1 ring complex interfaces and the Dam1 staple explains how kinetochore-microtubule attachments are destabilized and reset.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Saccharomyces cerevisiae
/
Kinétochores
/
Protéines du cycle cellulaire
/
Protéines de Saccharomyces cerevisiae
/
Protéines associées aux microtubules
/
Microtubules
Langue:
En
Journal:
Science
Année:
2023
Type de document:
Article
Pays d'affiliation:
Royaume-Uni