GCLiPP: global crosslinking and protein purification method for constructing high-resolution occupancy maps for RNA binding proteins.
Genome Biol
; 24(1): 281, 2023 Dec 07.
Article
de En
| MEDLINE
| ID: mdl-38062486
GCLiPP is a global RNA interactome capture method that detects RNA-binding protein (RBP) occupancy transcriptome-wide. GCLiPP maps RBP-occupied sites at a higher resolution than phase separation-based techniques. GCLiPP sequence tags correspond with known RBP binding sites and are enriched for sites detected by RBP-specific crosslinking immunoprecipitation (CLIP) for abundant cytosolic RBPs. Comparison of human Jurkat T cells and mouse primary T cells uncovers shared peaks of GCLiPP signal across homologous regions of human and mouse 3' UTRs, including a conserved mRNA-destabilizing cis-regulatory element. GCLiPP signal overlapping with immune-related SNPs uncovers stabilizing cis-regulatory regions in CD5, STAT6, and IKZF1.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Protéines de liaison à l'ARN
/
Transcriptome
Limites:
Animals
/
Humans
Langue:
En
Journal:
Genome Biol
Sujet du journal:
BIOLOGIA MOLECULAR
/
GENETICA
Année:
2023
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique
Pays de publication:
Royaume-Uni