lncRNA-Gm5532 regulates osteoclast differentiation through the miR-125a-3p/TRAF6 axis.
Acta Biochim Biophys Sin (Shanghai)
; 56(1): 54-61, 2024 01 25.
Article
de En
| MEDLINE
| ID: mdl-38098360
ABSTRACT
Long noncoding RNAs (lncRNAs) are important regulators of bone metabolism. In this study, lncRNA microarray analysis was used to identify differentially expressed lncRNAs in differentiated osteoclasts. lncRNA-Gm5532 is highly expressed during osteoclast differentiation. lncRNA-Gm5532 knockdown impairs osteoclast formation and bone resorption. Mechanistic experiments show that lncRNA-Gm5532 functions as a competing endogenous RNA (ceRNA) and acts as a sponge for miR-125a-3p, which promotes TNF receptor-associated factor 6 (TRAF6) expression. miR-125a-3p mimics suppress osteoclast differentiation and TAK1/NF-κB/MAPK signaling. The miR-125a-3p inhibitor reverses the negative effects of siGm5532 on osteoclast differentiation. In summary, our study reveals that lncRNA-Gm5532 functions as an activator in osteoclast differentiation by targeting the miR-125a-3p/TRAF6 axis, making it a novel biomarker and potential therapeutic target for osteoporosis.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Résorption osseuse
/
MicroARN
/
ARN long non codant
Limites:
Humans
Langue:
En
Journal:
Acta Biochim Biophys Sin (Shanghai)
Sujet du journal:
BIOFISICA
/
BIOQUIMICA
Année:
2024
Type de document:
Article
Pays de publication:
Chine