Enhancing ferryl accumulation in H2O2-dependent cytochrome P450s.
J Inorg Biochem
; 252: 112458, 2024 03.
Article
de En
| MEDLINE
| ID: mdl-38141432
ABSTRACT
A facile strategy is presented to enhance the accumulation of ferryl (iron(IV)-oxo) species in H2O2 dependent cytochrome P450s (CYPs) of the CYP152 family. We report the characterization of a highly chemoselective CYP decarboxylase from Staphylococcus aureus (OleTSA) that is soluble at high concentrations. Examination of OleTSA Compound I (CpdI) accumulation with a variety of fatty acid substrates reveals a dependence on resting spin-state equilibrium. Alteration of this equilibrium through targeted mutagenesis of the proximal pocket favors the high-spin form, and as a result, enhances Cpd-I accumulation to nearly stoichiometric yields.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Cytochrome P-450 enzyme system
/
Peroxyde d'hydrogène
Langue:
En
Journal:
J Inorg Biochem
Année:
2024
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique