G-quadruplex in mitochondria as a possible biomarker for mitophagy detection.
Int J Biol Macromol
; 259(Pt 2): 129337, 2024 Feb.
Article
de En
| MEDLINE
| ID: mdl-38218296
ABSTRACT
Mitochondrial autophagy (mitophagy) is a key physiological process that maintains the homeostasis of mitochondrial quality and quantity. Monitoring mitophagy is of great significance for detecting cellular abnormalities and developing therapeutic drugs. However, there are still very few biomarkers specifically developed for monitoring mitophagy. Here, we propose for the first time that mitochondrial G-quadruplex may serve as a biomarker for mitophagy detection, and develope a fluorescent light-up probe AMTC to monitor mitophagy in live cells. During mitophagy, AMTC fluorescence is significantly enhanced, but once mitophagy is inhibited, its fluorescence immediately decreases. The fluorescence behavior of AMTC implicates an increase in the formation of mitochondrial G-quadruplex during mitophagy. This inference has also been supported by the other two G-quadruplex probes. Taken together, this work provides a new possible biomarker and detection tool for the study of mitophagy.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Mitophagie
/
Mitochondries
Type d'étude:
Diagnostic_studies
Langue:
En
Journal:
Int J Biol Macromol
Année:
2024
Type de document:
Article