Novel 3D-Printed Microfluidic Magnetic Platform for Rapid DNA Isolation.
Anal Chem
; 96(5): 1985-1992, 2024 02 06.
Article
de En
| MEDLINE
| ID: mdl-38254336
ABSTRACT
This study presents a novel miniaturized device as a 3D-printed microfluidic magnetic platform specifically designed to manipulate magnetic microparticles in a microfluidic chip for rapid deoxyribonucleic acid (DNA) isolation. The novel design enables the movement of the magnetic particles in the same or opposite directions with the flow or suspends them in continuous flow. A computational model was developed to assess the effectiveness of the magnetic manipulation of the particles. Superparamagnetic monodisperse silica particles synthesized in-house are utilized for the isolation of fish sperm DNA and human placenta DNA. It was demonstrated that the proposed platform can perform DNA isolation within 10 min with an isolation efficiency of 50% at optimum operating conditions.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Microfluidique
/
Techniques d'analyse microfluidique
Limites:
Humans
/
Male
Langue:
En
Journal:
Anal Chem
Année:
2024
Type de document:
Article
Pays d'affiliation:
Turquie