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The dynamics of bacterial proliferation, viability, and extracellular polymeric substances in oral biofilm development.
He, Wendan; Liu, He; Wang, Zhejun; Tay, Franklin R; Shen, Ya.
Affiliation
  • He W; Department of Stomatology, The Affiliated Hospital of Wuhan Traditional Chinese and Western Medicine, Tongji Medical College of HUST, Wuhan 430022, China.
  • Liu H; Division of Endodontics, Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, 2199 Wesbrook Mall, Vancouver, BC V6T 1Z3, Canada.
  • Wang Z; Division of Endodontics, Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, 2199 Wesbrook Mall, Vancouver, BC V6T 1Z3, Canada.
  • Tay FR; Department of Endodontics, The Dental College of Georgia, Augusta University, Augusta, GA, USA. Electronic address: ftay@augusta.edu.
  • Shen Y; Division of Endodontics, Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, 2199 Wesbrook Mall, Vancouver, BC V6T 1Z3, Canada. Electronic address: yashen@dentistry.ubc.ca.
J Dent ; 143: 104882, 2024 04.
Article de En | MEDLINE | ID: mdl-38331378
ABSTRACT

OBJECTIVES:

This study investigated the relationship between bacterial growth, viability, and extracellular polymeric substances (EPS) formation in biofilms, particularly regarding resistance development. It also examined the impact of chemical factors on the EPS matrix and bacterial proliferation in oral biofilms.

METHODS:

Three multi-species oral biofilms were incubated in anaerobic conditions. Three strains of Enterococcus faecalis were incubated in aerobic conditions. The incubation periods ranged from 0 h to 7 days for short-term biofilms, and from 3 to 90 days for long-term biofilms. Fluorescent labeling with carboxyfluorescein diacetate succinimidyl ester (CFSE) and flow cytometry were used to track EPS and bacterial growth. Confocal laser scanning microscopy (CLSM) assessed bacterial viability and EPS structure. Biofilms aged 7, 14, and 21 days were treated with 2 % chlorhexidine (CHX) and 1 % sodium hypochlorite (NaOCl) to evaluate their effects on EPS and bacterial proliferation.

RESULTS:

Short-term biofilms showed rapid bacterial proliferation and a gradual increase in EPS, maintaining stable viability. In the first two weeks, a significant rise in CFSE indicated growing maturity. From 14 to 90 days, EPS and CFSE levels stabilized. Following treatment, CHX significantly reduced bacterial proliferation, while NaOCl decreased EPS volume.

CONCLUSIONS:

Biofilm development involves a balance between bacterial proliferation and EPS production. The complexity of this process poses challenges in treating biofilm-associated infections, requiring strategies tailored to the biofilm's developmental stage. CLINICAL

SIGNIFICANCE:

For effective root canal treatment, it is imperative to focus on reducing bacterial proliferation during the early stages of oral infections. In contrast, strategies aimed at minimizing EPS production could be more beneficial for long-term management of these conditions.
Sujet(s)
Mots clés

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Succinimides / Biofilms / Fluorescéines / Matrice de substances polymériques extracellulaires Langue: En Journal: J Dent Année: 2024 Type de document: Article Pays d'affiliation: Chine Pays de publication: Royaume-Uni

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Succinimides / Biofilms / Fluorescéines / Matrice de substances polymériques extracellulaires Langue: En Journal: J Dent Année: 2024 Type de document: Article Pays d'affiliation: Chine Pays de publication: Royaume-Uni