Role of macrophages in trigeminal ganglia in ectopic orofacial pain associated with pulpitis.
J Oral Biosci
; 66(1): 145-150, 2024 Mar.
Article
de En
| MEDLINE
| ID: mdl-38342297
ABSTRACT
OBJECTIVES:
This study aimed to elucidate the role of macrophages in the trigeminal ganglia (TG) in developing pulpitis-associated ectopic orofacial pain.METHODS:
Rats underwent maxillary pulp exposure, and Fluoro-Gold (FG) was administered in the ipsilateral whisker pad (WP). Head withdrawal threshold (HWT) upon mechanical stimulation of the WP was recorded, and liposomal clodronate clophosome-A (LCCA; macrophage depletion agent) was administered to the TG at three and four days after pulp exposure. Immunohistochemically, TG sections were stained with anti-Iba1 (a macrophage marker) and anti-Nav1.7 antibodies.RESULTS:
Pulp exposure decreased HWT and increased the number of Iba1-IR cells near FG-labelled TG neurons. LCCA inhibited the decrease in HWT and stopped the increase of FG-labelled Nav1.7-IR TG neurons in the pulpitis group.CONCLUSIONS:
Activation of macrophages by pulpitis induces the overexpression of Nav1.7 in TG neurons receiving inputs from WP, resulting in pulpitis-induced ectopic facial mechanical allodynia.Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Pulpite
Type d'étude:
Risk_factors_studies
Limites:
Animals
Langue:
En
Journal:
J Oral Biosci
/
J. oral biosci. (Online)
/
Journal of oral biosciences (Online)
Année:
2024
Type de document:
Article
Pays d'affiliation:
Japon
Pays de publication:
Pays-Bas