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Relevance of lymphocyte proliferation to PHA in severe combined immunodeficiency (SCID) and T cell lymphopenia.
Abraham, Roshini S; Basu, Amrita; Heimall, Jennifer R; Dunn, Elizabeth; Yip, Alison; Kapadia, Malika; Kapoor, Neena; Satter, Lisa Forbes; Buckley, Rebecca; O'Reilly, Richard; Cuvelier, Geoffrey D E; Chandra, Sharat; Bednarski, Jeffrey; Chaudhury, Sonali; Moore, Theodore B; Haines, Hilary; Dávila Saldaña, Blachy J; Chellapandian, Deepakbabu; Rayes, Ahmad; Chen, Karin; Caywood, Emi; Chandrakasan, Shanmuganathan; Lugt, Mark Thomas Vander; Ebens, Christen; Teira, Pierre; Shereck, Evan; Miller, Holly; Aquino, Victor; Eissa, Hesham; Yu, Lolie C; Gillio, Alfred; Madden, Lisa; Knutsen, Alan; Shah, Ami J; DeSantes, Kenneth; Barnum, Jessie; Broglie, Larisa; Joshi, Avni Y; Kleiner, Gary; Dara, Jasmeen; Prockop, Susan; Martinez, Caridad; Mousallem, Talal; Oved, Joseph; Burroughs, Lauri; Marsh, Rebecca; Torgerson, Troy R; Leiding, Jennifer W; Pai, Sung Yun; Kohn, Donald B.
Affiliation
  • Abraham RS; Department of Pathology and Laboratory Medicine, Nationwide Children's Hospital, OH, USA. Electronic address: Roshini.Abraham@nationwidechildrens.org.
  • Basu A; Department of Pathology and Laboratory Medicine, Nationwide Children's Hospital, OH, USA.
  • Heimall JR; Division of Allergy and Immunology, Children's Hospital of Philadelphia, PA, USA.
  • Dunn E; Division of Allergy, Immunology, and Blood and Marrow Transplantation, Department of Pediatrics, University of California San Francisco, San Francisco, CA, USA.
  • Yip A; Division of Allergy, Immunology, and Blood and Marrow Transplantation, Department of Pediatrics, University of California San Francisco, San Francisco, CA, USA.
  • Kapadia M; Division of Pediatric Oncology, Dana-Farber/Boston Children's Cancer and Blood Disorders Center, Department of Pediatrics, Harvard University Medical School, Boston, MA, USA.
  • Kapoor N; Transplantation and Cellular Therapy Program, Cancer and Blood Disease Institute, Children's Hospital Los Angeles, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
  • Satter LF; Pediatrics, Immunology, Allergy and Rheumatology, Baylor College of Medicine, Houston, TX, USA.
  • Buckley R; Departments of Pediatrics and Immunology, Duke University Medical Center, Durham, NC, USA.
  • O'Reilly R; Department of Pediatrics, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
  • Cuvelier GDE; Manitoba Blood and Marrow Transplant Program, CancerCare Manitoba, Department of Pediatrics and Child Health, University of Manitoba, Winnipeg, MB, Canada.
  • Chandra S; Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, USA; Division of Bone Marrow Transplantation and Immune Deficiency, Cancer and Blood Disease Institute, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
  • Bednarski J; Division of Hematology and Oncology, Department of Pediatrics, Washington University School of Medicine, St. Louis, MO, USA.
  • Chaudhury S; Division of Hematology, Oncology, and Stem Cell Transplantation, Ann & Robert H. Lurie Children's Hospital of Chicago-Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
  • Moore TB; Division of Hematology/Oncology, Mattel Children's Hospital at UCLA, Los Angeles, CA, USA.
  • Haines H; Division of Pediatric Hematology-Oncology and Bone Marrow Transplant, University of Alabama at Birmingham, Birmingham, AL, USA.
  • Dávila Saldaña BJ; Division of Blood and Marrow Transplantation, Children's National Hospital-George Washington University School of Medicine and Health Sciences, Washington, DC, USA.
  • Chellapandian D; Blood and Marrow Transplant, Johns Hopkins All Children's Hospital, St. Petersburg, FL, USA.
  • Rayes A; Division of Pediatric Hematology and Oncology, Intermountain Primary Childrens Hospital, Huntsman Cancer Institute, Spencer Fox Eccles School of Medicine at the University of Utah, Salt Lake City, UT, USA.
  • Chen K; Department of Pediatrics, University of Washington-Seattle Children's Hospital, Seattle, WA, USA.
  • Caywood E; Nemours Children's Health Delaware, Thomas Jefferson University, Wilmington, DE, USA.
  • Chandrakasan S; Bone Marrow Transplantation Program, Aflac Cancer and Blood Disorders Center, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, USA.
  • Lugt MTV; Blood and Marrow Transplant Program, University of Michigan, Ann Arbor, MI, USA.
  • Ebens C; Division of Pediatric Blood and Marrow Transplantation & Cellular Therapy, University of Minnesota, Minneapolis, MN, USA.
  • Teira P; Pediatric Immunology and Rheumatology Division, CHU Sainte-Justine, Department of Pediatrics, University of Montreal, Montreal, QC, Canada.
  • Shereck E; Division of Pediatric Hematology/Oncology, Oregon Health and Science University, Portland, OR, USA.
  • Miller H; Phoenix Children's Hospital, Phoenix, AZ, USA.
  • Aquino V; Division of Pediatric Hematology, Oncology and Stem Cell Transplantation, University of Texas Southwestern Medical Center, Dallas, TX, USA.
  • Eissa H; Division of Pediatric Hematology-Oncology-BMT, University of Colorado, Aurora, CO, USA.
  • Yu LC; Division of Pediatric Hematology-Oncology/HSCT, LSUHSC and Children's Hospital, New Orleans, LA, USA.
  • Gillio A; Institute for Pediatric Cancer and Blood Disorders, Hackensack University Medical Center, Hackensack, NJ, USA.
  • Madden L; Pediatric Blood and Marrow Transplantation Program, Texas Transplant Institute, Methodist Children's Hospital, San Antonio, TX, USA.
  • Knutsen A; Department of Pediatrics, Pediatric Allergy and Immunology Division, Saint Louis University, St Louis, MO, USA.
  • Shah AJ; Division of Stem Cell Transplantation and Regenerative Medicine, Department of Pediatrics, Stanford School of Medicine Pediatric Stem Cell Transplantation, Stanford University, Stanford, CA, USA.
  • DeSantes K; American Family Children's Hospital, University of Wisconsin, Madison, WI, USA.
  • Barnum J; UPMC Children's Hospital of Pittsburgh, Pittsburgh, PA, USA.
  • Broglie L; Division of Pediatric Hematology, Oncology, and Blood and Marrow Transplantation, Medical College of Wisconsin, Milwaukee, WI, USA.
  • Joshi AY; Division of Pediatric and Adult Allergy and Immunology, Mayo Clinic, Rochester, MN, USA.
  • Kleiner G; Division of Pediatric Allergy and Immunology, Department of Pediatrics, Holtz Children's Hospital at Jackson Memorial Hospital, University of Miami Miller School of Medicine, Miami, FL, USA.
  • Dara J; Division of Allergy, Immunology, and Blood and Marrow Transplantation, Department of Pediatrics, University of California San Francisco, San Francisco, CA, USA.
  • Prockop S; Division of Pediatric Oncology, Dana-Farber/Boston Children's Cancer and Blood Disorders Center, Department of Pediatrics, Harvard University Medical School, Boston, MA, USA.
  • Martinez C; Pediatrics, Immunology, Allergy and Rheumatology, Baylor College of Medicine, Houston, TX, USA.
  • Mousallem T; Departments of Pediatrics and Immunology, Duke University Medical Center, Durham, NC, USA.
  • Oved J; Department of Pediatrics, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
  • Burroughs L; Department of Pediatrics, University of Washington-Seattle Children's Hospital, Seattle, WA, USA.
  • Marsh R; Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, USA; Division of Bone Marrow Transplantation and Immune Deficiency, Cancer and Blood Disease Institute, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA; Pharming Healthcare Inc, Warren, NJ, USA
  • Torgerson TR; Department of Pediatrics, University of Washington-Seattle Children's Hospital, Seattle, WA, USA.
  • Leiding JW; Division of Allergy and Immunology, Department of Pediatrics, Johns Hopkins University Baltimore, MD and Institute for Clinical and Translational Research, Johns Hopkins All Childrens Hospital, St. Petersburg, FL, USA.
  • Pai SY; Immune Deficiency Cellular Therapy Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
  • Kohn DB; Department of Pediatrics, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA, USA.
Clin Immunol ; 261: 109942, 2024 04.
Article de En | MEDLINE | ID: mdl-38367737
ABSTRACT
Severe combined immunodeficiency (SCID) is characterized by a severe deficiency in T cell numbers. We analyzed data collected (n = 307) for PHA-based T cell proliferation from the PIDTC SCID protocol 6901, using either a radioactive or flow cytometry method. In comparing the two groups, a smaller number of the patients tested by flow cytometry had <10% of the lower limit of normal proliferation as compared to the radioactive method (p = 0.02). Further, in patients with CD3+ T cell counts between 51 and 300 cells/µL, there was a higher proliferative response with the PHA flow assay compared to the 3H-T assay (p < 0.0001), suggesting that the method of analysis influences the resolution and interpretation of PHA results. Importantly, we observed many SCID patients with profound T cell lymphopenia having normal T cell proliferation when assessed by flow cytometry. We recommend this test be considered only as supportive in the diagnosis of typical SCID.
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Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Immunodéficience combinée grave / Lymphopénie Limites: Humans / Newborn Langue: En Journal: Clin Immunol Sujet du journal: ALERGIA E IMUNOLOGIA Année: 2024 Type de document: Article
Texte intégral
Ajouter à My VHL
Imprimer
XML
PubMed Links
Recherche sur Google

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Immunodéficience combinée grave / Lymphopénie Limites: Humans / Newborn Langue: En Journal: Clin Immunol Sujet du journal: ALERGIA E IMUNOLOGIA Année: 2024 Type de document: Article