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Medical prospects of cryptosporidiosis in vivo control using biofabricated nanoparticles loaded with Cinnamomum camphora extracts by Ulva fasciata.
Allam, Nesreen Allam Tantawy; Hamouda, Ragaa Abd El-Fatah; Sedky, Doaa; Abdelsalam, Mahinour Ezzeldin; El-Gawad, Mona Ebrahim Hussien Abd; Hassan, Noha Mahmoud Fahmy; Aboelsoued, Dina; Elmaaty, Amal M Abou; Ibrahim, Muhammad A; Taie, Hanan Anwar Aly; Hakim, Ashraf Samir; Desouky, Hassan Mohamed; Megeed, Kadria Nasr Abdel; Abdel-Hamid, Marwa Salah.
Affiliation
  • Allam NAT; Department of Parasitology and Animal Diseases, Veterinary Research Institute, National Research Centre, 33 El Buhouth Street, Dokki, P.O. Box: 12622, Giza, Cairo, Egypt.
  • Hamouda RAE; Department of Microbial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, 5 Zone, Sadat City, Munofia, Egypt.
  • Sedky D; Department of Parasitology and Animal Diseases, Veterinary Research Institute, National Research Centre, 33 El Buhouth Street, Dokki, P.O. Box: 12622, Giza, Cairo, Egypt.
  • Abdelsalam ME; Department of General Biology, Center of Basic Sciences, Misr University for Science and Technology, Al Motamayez District, 6 of October, Giza, Cairo, Egypt.
  • El-Gawad MEHA; Cytogenetics and Animal Cell Culture Lab., National Gene Bank, Agriculture Research Center, 9 Gamaa Street, Giza, Cairo, Egypt.
  • Hassan NMF; Department of Parasitology and Animal Diseases, Veterinary Research Institute, National Research Centre, 33 El Buhouth Street, Dokki, P.O. Box: 12622, Giza, Cairo, Egypt.
  • Aboelsoued D; Department of Parasitology and Animal Diseases, Veterinary Research Institute, National Research Centre, 33 El Buhouth Street, Dokki, P.O. Box: 12622, Giza, Cairo, Egypt.
  • Elmaaty AMA; Department of Animal Reproduction and Artificial Insemination, Veterinary Research Institute, National Research Centre, 33 El Buhouth Street, Dokki, P.O. Box: 12622, Giza, Cairo, Egypt.
  • Ibrahim MA; Cytogenetics and Animal Cell Culture Lab., National Gene Bank, Agriculture Research Center, 9 Gamaa Street, Giza, Cairo, Egypt.
  • Taie HAA; Department of Plant Biochemistry, Agriculture and Biological Researches Institute, National Research Centre, 33 El-Bohouth St. (Former El-Tahrir St.), Dokki, P.O. 12622, Giza, Cairo, Egypt.
  • Hakim AS; Department of Microbiology and Immunology, Veterinary Research Institute, National Research Centre, 33 El Buhouth Street, Dokki, P.O. Box: 12622, Giza, Cairo, Egypt.
  • Desouky HM; Department of Animal Reproduction and Artificial Insemination, Veterinary Research Institute, National Research Centre, 33 El Buhouth Street, Dokki, P.O. Box: 12622, Giza, Cairo, Egypt.
  • Megeed KNA; Department of Parasitology and Animal Diseases, Veterinary Research Institute, National Research Centre, 33 El Buhouth Street, Dokki, P.O. Box: 12622, Giza, Cairo, Egypt.
  • Abdel-Hamid MS; Department of Microbial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, 5 Zone, Sadat City, Munofia, Egypt.
Vet World ; 17(1): 108-124, 2024 Jan.
Article de En | MEDLINE | ID: mdl-38406364
ABSTRACT
Background and

Aim:

Global efforts are continuing to develop preparations against cryptosporidiosis. This study aimed to investigate the efficacy of biosynthesized Ulva fasciata loading Cinnamomum camphora oil extract on new zinc oxide nanoparticles (ZnONPs shorten to ZnNPs) and silver nanoparticles (AgNPs) as alternative treatments for Cryptosporidium parvum experimental infection in rats. Materials and

Methods:

Oil extract was characterized by gas chromatography-mass spectrometry, loaded by U. fasciata on ionic-based ZnO and NPs, and then characterized by transmission electron microscopy, scanning electron microscopy, and X-ray diffraction. Biosafety and toxicity were investigated by skin tests. A total of 105 C. parvum oocysts/rat were used (n = 81, 2-3 W, 80-120 g, 9 male rats/group). Oocysts shedding was counted for 21 d. Doses of each preparation in addition to reference drug were administered daily for 7 d, starting on post-infection (PI) day (3). Nitazoxanide (100 mg) was used as the reference drug. After 3 weeks, the rats were sacrificed for postmortem examination and histopathological examination. Two blood samples/rat/group were collected on the 21st day. Ethylenediaminetetraacetic acid blood samples were also used for analysis of biochemistry, hematology, immunology, micronucleus prevalence, and chromosomal abnormalities.

Results:

C. camphora leaves yielded 28.5 ± 0.3 g/kg oil and 20 phycocompounds were identified. Spherical and rod-shaped particles were detected at 10.47-30.98 nm and 18.83-38.39 nm, respectively. ZnNPs showed the earliest anti-cryptosporidiosis effect during 7-17 d PI. Other hematological, biochemical, immunological, histological, and genotoxicity parameters were significantly fruitful; hence, normalized pathological changes induced by infestation were observed in the NPs treatments groups against the infestation-free and Nitazoxanide treated group.

Conclusion:

C. camphora, U. fasciata, ZnNPs, and AgNPs have refluxed the pathological effects of infection as well as positively improved host physiological condition by its anticryptosporidial immunostimulant regenerative effects with sufficient ecofriendly properties to be proposed as an alternative to traditional drugs, especially in individuals with medical reactions against chemical commercial drugs.
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Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Langue: En Journal: Vet World Année: 2024 Type de document: Article Pays d'affiliation: Égypte
Texte intégral
Ajouter à My VHL
Imprimer
XML
PubMed Links
Recherche sur Google

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Langue: En Journal: Vet World Année: 2024 Type de document: Article Pays d'affiliation: Égypte