Investigation into effects of tocilizumab and epoetin beta in rats with experimental sciatic nerve injury model.
Tissue Cell
; 88: 102357, 2024 Jun.
Article
de En
| MEDLINE
| ID: mdl-38493757
ABSTRACT
OBJECTIVE:
To investigate the effects of tocilizumab (TCZ), epoetin beta (EPO), and their combination on nerve regeneration in a sciatic nerve injury model. MATERIALS ANDMETHOD:
Male Sprague-Dawley rats were divided into (-) negative control, sham, TCZ, EPO ((+) positive control), and TCZ+EPO groups. The TCZ group received TCZ (8â¯mg/kg intraperitoneal) immediately after surgery. On day 14th, the EPO group received EPO (5000 IU/kg, intraperitoneal); the TCZ+EPO group received TCZ (8â¯mg/kg, intraperitoneal), EPO (5000 IU/kg, intraperitoneal), and TCZ (8â¯mg/kg, intraperitoneal) post-surgery. Motor and sensory functions were assessed pre and post-surgery. Lipid peroxidation and oxidative stress parameters were evaluated biochemically in the serum, and sciatic nerve tissue was evaluated histopathologically using haematoxylin-Eosin and Masson trichrome staining.CONCLUSIONS:
TCZ and EPO decreased nerve injury effects by increasing motor and sensory conduction velocities of the sciatic nerve. Biochemically, TCZ and EPO significantly increased Superoxide Dismutase, Catalase, and Glutathione peroxidase 4 levels while decreasing Lipid Peroxidation levels (p=0.001). Histopathologically, neuronal degeneration following nerve injury was decreased in the groups receiving TCZ and EPO (p=0.001). EPO and TCZ attenuate the adverse effects of nerve injury. However, the TCZ+EPO treatment favoured biochemical activities over tissue and functional activities. This has been confirmed functionally, biochemically, and histopathologically.Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Nerf ischiatique
/
Érythropoïétine
/
Rat Sprague-Dawley
/
Modèles animaux de maladie humaine
/
Anticorps monoclonaux humanisés
Limites:
Animals
Langue:
En
Journal:
Tissue Cell
Année:
2024
Type de document:
Article
Pays de publication:
Royaume-Uni