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Microbulbifer magnicolonia sp. nov., isolated from sediment of tidal flat located in Zhoushan, China.
Tao, Lang-Ci; Gao, Jia-Wei; Su, Yue; Zhang, Wen-Wu; Cao, Ke; Yu, Xiao-Yun; Ma, Kuo-Jian; Xu, Lin; Xia, Xiao-Ming; Sun, Cong.
Affiliation
  • Tao LC; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, PR China.
  • Gao JW; Shaoxing Biomedical Research Institute of Zhejiang Sci-Tech University Co., Ltd, Zhejiang Engineering Research Center for the Development Technology of Medicinal and Edible Homologous Health Food, Shaoxing 312075, PR China.
  • Su Y; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, PR China.
  • Zhang WW; Shaoxing Biomedical Research Institute of Zhejiang Sci-Tech University Co., Ltd, Zhejiang Engineering Research Center for the Development Technology of Medicinal and Edible Homologous Health Food, Shaoxing 312075, PR China.
  • Cao K; Trend Biotech Co., Ltd., Hangzhou 311121, PR China.
  • Yu XY; Trend Biotech Co., Ltd., Hangzhou 311121, PR China.
  • Ma KJ; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, PR China.
  • Xu L; Department of Clinical Laboratory, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou 310004, PR China.
  • Xia XM; Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou 310012, PR China.
  • Sun C; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, PR China.
Article de En | MEDLINE | ID: mdl-38546457
ABSTRACT
A Gram-stain-negative, aerobic, motile with flagella and rod- or ovoid-shaped bacterium, designated GG15T, was isolated from tidal flat sediment sampled in Zhoushan, Zhejiang Province. Strain GG15T grew at 20-40 °C (optimum, 30 °C), at pH 5.5-9.5 (optimum, pH 7.0-8.0) and with 1.0-10.0 % (w/v) NaCl (optimum, 1.5 %). Colony diameters ranged from 1 to 3 mm within the first week, reaching a maximum of 6-7 mm after 15 days of cultivation. Strain GG15T exhibited highest 16S rRNA gene sequence similarity to Microbulbifer taiwanensis CCM 7856T (98.1 %), with similarity to other species within the genus Microbulbifer ranging from 97.8 to 93.8 %. Similarity values to other genera were below 93.8 %. Strain GG15T exhibited positive activity for ß-glucosidase, trypsin and chymotrypsin, whereas the reference strain showed negative activity. Chemotaxonomic analyses indicated that strain GG15T contained Q-8 as the sole respiratory quinone, C16  0 (9.1 %), iso-C15  0 (30.9 %) and iso-C11  0 3-OH (7.2 %) as the predominant fatty acids, and phosphatidylethanolamine, phosphatidylglycerol, three unidentified lipids, four unidentified glycolipids, one unidentified phospholipid, two unidentified aminolipids and two unidentified aminophospholipids as the main polar lipids. The genome of strain GG15T was 4 307 641 bp long, comprising 3861 protein-coding genes. The G+C content of strain GG15T was 61.5 mol% based on its genomic sequence. Strain GG15T showed low digital DNA-DNA hybridization (<70 %) and average nucleotide identity values (<95 %) with other Microbulbifer species. As a result, a novel species within the genus Microbulbifer, named Microbulbifer magnicolonia sp. nov., is proposed. The type strain is GG15T (MCCC 1K08802T=KCTC 8210T).
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Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Alteromonadaceae / Acides gras Pays/Région comme sujet: Asia Langue: En Journal: Int J Syst Evol Microbiol Sujet du journal: MICROBIOLOGIA Année: 2024 Type de document: Article Pays de publication: Royaume-Uni

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Alteromonadaceae / Acides gras Pays/Région comme sujet: Asia Langue: En Journal: Int J Syst Evol Microbiol Sujet du journal: MICROBIOLOGIA Année: 2024 Type de document: Article Pays de publication: Royaume-Uni