The virome of the kitome: small circular virus-like genomes in laboratory reagents.

Duan, Jiayi; Keeler, Emma; McFarland, Alexander; Scott, Phillip; Collman, Ronald G; Bushman, Frederic D

Duan, Jiayi; Keeler, Emma; McFarland, Alexander; Scott, Phillip; Collman, Ronald G; Bushman, Frederic D.

Affiliation

Duan J; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
Keeler E; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
McFarland A; Department of Microbial Pathogenesis, Yale University School of Medicine, New Haven, Connecticut, USA.
Scott P; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
Collman RG; Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
Bushman FD; Pulmonary and Critical Care Division, Department of Medicine, Center for Translational Lung Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA.

Microbiol Resour Announc ; : e0126123, 2024 Apr 09.

Article de En | MEDLINE | ID: mdl-38591883

ABSTRACT

ABSTRACT

In the course of studying the virome of protozoan parasites, we identified small circular genomes resembling viruses, which turned out to be contaminants from an RNA purification kit. We report their sequences here so others can detect possible contamination in their samples by aligning them to these targets.

Mots clés

contamination; genome; kitome; virome; virus

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Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Langue: En Journal: Microbiol Resour Announc Année: 2024 Type de document: Article Pays d'affiliation: États-Unis d'Amérique

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