Imaging extrachromosomal DNA (ecDNA) in cancer.
Histochem Cell Biol
; 162(1-2): 53-64, 2024 Jul.
Article
de En
| MEDLINE
| ID: mdl-38625562
ABSTRACT
Extrachromosomal DNA (ecDNA) are circular regions of DNA that are found in many cancers. They are an important means of oncogene amplification, and correlate with treatment resistance and poor prognosis. Consequently, there is great interest in exploring and targeting ecDNA vulnerabilities as potential new therapeutic targets for cancer treatment. However, the biological significance of ecDNA and their associated regulatory control remains unclear. Light microscopy has been a central tool in the identification and characterisation of ecDNA. In this review we describe the different cellular models available to study ecDNA, and the imaging tools used to characterise ecDNA and their regulation. The insights gained from quantitative imaging are discussed in comparison with genome sequencing and computational approaches. We suggest that there is a crucial need for ongoing innovation using imaging if we are to achieve a full understanding of the dynamic regulation and organisation of ecDNA and their role in tumourigenesis.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Tumeurs
Limites:
Humans
Langue:
En
Journal:
Histochem Cell Biol
Sujet du journal:
CITOLOGIA
/
HISTOCITOQUIMICA
Année:
2024
Type de document:
Article
Pays d'affiliation:
Royaume-Uni