Biocompatibility and acid resistance of preformed crowns in children: an in vitro study.
Eur Arch Paediatr Dent
; 25(3): 417-425, 2024 Jun.
Article
de En
| MEDLINE
| ID: mdl-38662172
ABSTRACT
PURPOSE:
To investigate the in vitro biocompatibility of human gingival fibroblasts with preformed paediatric crowns and resistance to acid exposure at levels that simulate the oral environment.METHODS:
This laboratory study investigated primary HGFs viability, metabolic activity, cytotoxicity, and apoptotic events on preformed metal crown discs, composite resin-coated wells, and monolithic zirconia fragments at 24, 48, and 72 h using the ApoTox-Glo Triplex assay. The PPCs were also immersed in 0.1% lactic acid, 0.2% phosphoric acid, or 10% citric acid for 7 days at 37 °C to reproduce conditions associated with dietary intake or gastric reflux. Samples were then subject to inductively coupled plasma optical emission spectrometry to quantitate the release of ions.RESULTS:
The viability of HGFs on stainless steel and CR significantly declined at 48 and 72 h, representing potential cytotoxicity (p < 0.05). Cytotoxicity of HGFs was also higher for stainless steel and ZR compared to control (p < 0.05). PMCs and ZR crowns gave minimal ion release. Meanwhile, significant quantities of metallic ions, including copper (Cu), iron (Fe), nickel (Ni), and zinc (Zn), were present in eluates from veneered-preformed metal crowns.CONCLUSION:
As PPCs can be exposed to highly acidic environments for many years, thus the release of metallic ions from V-PMCs should form the further investigation in future studies.Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Acier inoxydable
/
Zirconium
/
Matériaux biocompatibles
/
Test de matériaux
/
Couronnes
/
Fibroblastes
/
Gencive
Limites:
Child
/
Humans
Langue:
En
Journal:
Eur Arch Paediatr Dent
Sujet du journal:
ODONTOLOGIA
/
PEDIATRIA
Année:
2024
Type de document:
Article
Pays d'affiliation:
Australie