Wide temperature adaptive oxidase-like based on mesoporous manganese based metal-organic framework for detecting total antioxidant capacity.
Food Chem
; 451: 139378, 2024 Sep 01.
Article
de En
| MEDLINE
| ID: mdl-38670019
ABSTRACT
Overcoming the intense variation of enzymatic activity among different temperatures is very critical in catalytic medicine and catalytic biology. Here, Mn-based metal-organic framework-based wide-temperature-adaptive mesoporous artificial enzymes (Mn-TMA-MOF) were designed and synthesized. The oxidase-like Mn-TMA-MOF showed excellent catalytic activity at 0-50 °C and avoided the activity loss and instability due to temperature variation that occurred. The excellent oxidase-like properties of Mn-TMA-MOF with wide temperature adaptativeness are mainly ascribed to the mixed oxidized state (Mn3+/Mn2+) and high substrate affinity (Km = 0.034 mM) of Mn. Moreover, the mesopore-micropores two-level structure of Mn-TMA-MOF provides a large space and surface area for enzyme catalysis. Based on the stability of Mn-TMA-MOF, we developed a colorimetric sensor that can detect total antioxidant capacity in fruits with a limit of detection up to 0.59 µM.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Oxidoreductases
/
Température
/
Réseaux organométalliques
/
Manganèse
/
Antioxydants
Langue:
En
Journal:
Food Chem
Année:
2024
Type de document:
Article
Pays de publication:
Royaume-Uni