[Expression changes of RNA m6A regulators in mouse cerebellum affected by hypobaric hypoxia stimulation].
Zhonghua Bing Li Xue Za Zhi
; 53(5): 452-457, 2024 May 08.
Article
de Zh
| MEDLINE
| ID: mdl-38678325
ABSTRACT
Objective:
To investigate the role of RNA m6A methylation in mediating cerebellar dysplasia through analyzing the phenotypes of the mouse cerebella and the expression of several key m6A regulators upon hypobaric hypoxia treatment.Methods:
Five-day old C57/BL6 mice were exposed to hypobaric hypoxia for 9 days. The status of mouse cerebellar development was analyzed by comparing the body weights, brain weights and histological features. Immunostaining of cell-type-specific markers was performed to analyze the cerebellar morphology. Real-time PCR, Western blot and immunohistochemical staining were performed to detect the expression of key m6A regulators in the mouse cerebella.Results:
Compared with the control, the body weights, brain weights and cerebellar volumes of hypobaric hypoxic mice were significantly reduced (P<0.01). The expression of specific markers in different cells, including NeuN (mature neuron), Calbindin-D28K (Purkinje cell) and GFAP (astrocyte), was decreased in hypobaric hypoxic mouse cerebella (P<0.01), accompanied with disorganized cellular structure. The expression of methyltransferase METTL3 was significantly down-regulated in the cerebella of hypobaric hypoxic mice (P<0.05).Conclusions:
Hypobaric hypoxia stimulation causes mouse cerebellar dysplasia, with structural abnormalities in mature granular neurons, Purkinje cells and astrocytes. Expression of METTL3 is decreased in hypobaric hypoxic mice cerebellum compared with that of normobaric normoxic mice, suggesting that its mediated RNA m6A methylation may play an important role in hypobaric hypoxia-induced mouse cerebellar dysplasia.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Cellules de Purkinje
/
Cervelet
/
Protéines de liaison à l'ADN
/
Calbindines
/
Hypoxie
/
Methyltransferases
/
Souris de lignée C57BL
/
Protéines de tissu nerveux
Limites:
Animals
Langue:
Zh
Journal:
Zhonghua Bing Li Xue Za Zhi
Année:
2024
Type de document:
Article
Pays d'affiliation:
Chine