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[α2-macroglobulin alleviates glucocorticoid-induced avascular necrosis of the femoral head in mice by promoting proliferation, migration and angiogenesis of vascular endothelial cells].
Zhu, Q; Lu, Y; Peng, Y; He, J; Wei, Z; Li, Z; Chen, Y.
Affiliation
  • Zhu Q; Department of Joint Surgery, Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510630, China.
  • Lu Y; Department of Joint Surgery, Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510630, China.
  • Peng Y; Department of Joint Surgery, Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510630, China.
  • He J; Department of Joint Surgery, Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510630, China.
  • Wei Z; Department of Joint Surgery, Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510630, China.
  • Li Z; Department of Joint Surgery, Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510630, China.
  • Chen Y; Department of Joint Surgery, Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510630, China.
Nan Fang Yi Ke Da Xue Xue Bao ; 44(4): 712-719, 2024 Apr 20.
Article de Zh | MEDLINE | ID: mdl-38708505
ABSTRACT

OBJECTIVE:

To explore the mechanism underlying the protective effect of α2-macroglobulin (A2M) against glucocorticoid-induced femoral head necrosis.

METHODS:

In a human umbilical vein endothelial cell (HUVEC) model with injuries induced by gradient concentrations of dexamethasone (DEX; 10-8-10-5 mol/L), the protective effects of A2M at 0.05 and 0.1 mg/mL were assessed by examining the changes in cell viability, migration, and capacity of angiogenesis using CCK-8 assay, Transwell and scratch healing assays and angiogenesis assay. The expressions of CD31 and VEGF-A proteins in the treated cells were detected using Western blotting. In BALB/c mouse models of avascular necrosis of the femoral head induced by intramuscular injections of methylprednisolone, the effects of intervention with A2M on femoral trabecular structure, histopathological characteristics, and CD31 expression were examined with Micro-CT, HE staining and immunohistochemical staining.

RESULTS:

In cultured HUVECs, DEX treatment significantly reduced cell viability, migration and angiogenic ability in a concentration- and time-dependent manner (P<0.05), and these changes were obviously reversed by treatment with A2M in positive correlation with A2M concentration (P<0.05). DEX significantly reduced the expression of CD31 and VEGF-A proteins in HUVECs, while treatment with A2M restored CD31 and VEGF-A expressions in the cells (P<0.05). The mouse models of femoral head necrosis showed obvious trabecular damages in the femoral head, where a large number of empty lacunae and hypertrophic fat cells could be seen and CD31 expression was significantly decreased (P<0.05). A2M treatment of the mouse models significantly improved trabecular damages, maintained normal bone tissue structures, and increased CD31 expression in the femoral head (P<0.05).

CONCLUSION:

A2M promotes proliferation, migration, and angiogenesis of DEX-treated HUVECs and alleviates methylprednisolone-induced femoral head necrosis by improving microcirculation damages and maintaining microcirculation stability in the femoral head.
Sujet(s)
Mots clés

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Dexaméthasone / Nécrose de la tête fémorale / Glucocorticoïdes Limites: Animals / Humans Langue: Zh Journal: Nan Fang Yi Ke Da Xue Xue Bao Année: 2024 Type de document: Article Pays d'affiliation: Chine

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Dexaméthasone / Nécrose de la tête fémorale / Glucocorticoïdes Limites: Animals / Humans Langue: Zh Journal: Nan Fang Yi Ke Da Xue Xue Bao Année: 2024 Type de document: Article Pays d'affiliation: Chine