Isolation, structural characterization and immunomodulatory activity on RAW264.7 cells of a novel exopolysaccharide of Dictyophora rubrovalvata.
Int J Biol Macromol
; 270(Pt 2): 132222, 2024 Jun.
Article
de En
| MEDLINE
| ID: mdl-38729468
ABSTRACT
Fungal polysaccharides have been explored by many for both structural studies and biological activities, but few studies have been done on the extracellular polysaccharides of Dictyophora rubrovalvata, so a new exopolysaccharide was isolated from Dictyophora rubrovalvata and its structure and its immunological activity were investigated. The crude exopolysaccharide (EPS) was purified by DEAE52 cellulose and Sephadex G-200 to obtain a new acidic polysaccharide (DR-EPS). DR-EPS (2.66â¯×â¯103â¯kDa) was consisted mainly of mannose, glucose, galactose and glucuronic acid with a molar ratio of 1 0.86 0.20 0.01. In addition, DR-EPS increased the phagocytic activity of RAW264.7 cells up to 2.67 times of the blank control group. DR-EPS improved intracellular nucleic acid and glycogen metabolism as observed by AO and PAS staining. DR-EPS(40⯵g/mL) promoted NO production up to 30.66⯵mol, enhanced acid phosphatase (ACP) and superoxide dismutase (SOD) activities, with activity maxima of 660â¯U/gprot and 96.27â¯U/mgprot, respectively, and DR-EPS (160⯵g / mL) significantly increased the lysozyme content as 2.73 times of the control group. The good immunological activity of extracellular polysaccharides of Dictyophora rubrovalvata provides directions for the use of fermentation broths.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Polysaccharides fongiques
Limites:
Animals
Langue:
En
Journal:
Int J Biol Macromol
Année:
2024
Type de document:
Article
Pays de publication:
Pays-Bas