The fungicide cymoxanil impairs respiration in Saccharomyces cerevisiae via cytochrome c oxidase inhibition.
FEBS Lett
; 598(13): 1655-1666, 2024 Jul.
Article
de En
| MEDLINE
| ID: mdl-38750637
ABSTRACT
Cymoxanil (CYM) is a widely used synthetic acetamide fungicide, but its biochemical mode of action remains elusive. Since CYM inhibits cell growth, biomass production, and respiration in Saccharomyces cerevisiae, we used this model to characterize the effect of CYM on mitochondria. We found it inhibits oxygen consumption in both whole cells and isolated mitochondria, specifically inhibiting cytochrome c oxidase (CcO) activity during oxidative phosphorylation. Based on molecular docking, we propose that CYM blocks the interaction of cytochrome c with CcO, hampering electron transfer and inhibiting CcO catalytic activity. Although other targets cannot be excluded, our data offer valuable insights into the mode of action of CYM that will be instrumental in driving informed management of the use of this fungicide.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Saccharomyces cerevisiae
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Complexe IV de la chaîne respiratoire
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Simulation de docking moléculaire
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Fongicides industriels
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Mitochondries
Langue:
En
Journal:
FEBS Lett
Année:
2024
Type de document:
Article
Pays d'affiliation:
Portugal
Pays de publication:
Royaume-Uni