Streptomyces beigongshangae sp. nov., isolated from baijiu fermented grains, could transform ginsenosides of Panax notoginseng.
Int J Syst Evol Microbiol
; 74(5)2024 May.
Article
de En
| MEDLINE
| ID: mdl-38767616
ABSTRACT
A Gram-stain-positive actinomycete, designated REN17T, was isolated from fermented grains of Baijiu collected from Sichuan, PR China. It exhibited branched substrate mycelia and a sparse aerial mycelium. The optimal growth conditions for REN17T were determined to be 28â°C and pH 7, with a NaCl concentration of 0â% (w/v). ll-Diaminopimelic acid was the diagnostic amino acid of the cell-wall peptidoglycan and the polar lipids were composed of phosphatidylethanolamine, phosphatidylinositol, an unidentified phospholipid, two unidentified lipids and four unidentified glycolipids. The predominant menaquinone was MK-9 (H2), MK-9 (H4), MK-9 (H6) and MK-9 (H8). The major fatty acids were iso-C16 0. The 16S rRNA sequence of REN17T was most closely related to those of Streptomyces apricus SUN 51T (99.8â%), Streptomyces liliiviolaceus BH-SS-21T (99.6â%) and Streptomyces umbirnus JCM 4521T (98.9â%). The digital DNA-DNA hybridization, average nucleotide identity and average amino acid identify values between REN17T and its closest replated strain, of S. apricus SUN 51T, were 35.9, 88.9 and 87.3â%, respectively. Therefore, REN17T represents a novel species within the genus Streptomyces, for which the name Streptomyces beigongshangae sp. nov. is proposed. The type strain is REN17T (=GDMCC 4.193T=JCM 34712T). While exploring the function of the strain, REN17T was found to possess the ability to transform major ginsenosides of Panax notoginseng (Burk.) F.H. Chen (Araliaceae) into minor ginsenoside through HPLC separation, which was due to the presence of ß-glucosidase. The recombinant ß-glucosidase was constructed and purified, which could produce minor ginsenosides of Rg3 and C-K. Finally, the enzymatic properties were characterized.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Phylogenèse
/
Streptomyces
/
ADN bactérien
/
ARN ribosomique 16S
/
Techniques de typage bactérien
/
Analyse de séquence d'ADN
/
Vitamine K2
/
Ginsénosides
/
Panax notoginseng
/
Acides gras
Pays/Région comme sujet:
Asia
Langue:
En
Journal:
Int J Syst Evol Microbiol
/
Int. j. syst. evol. microbiol
/
International journal of systematic and evolutionary microbiology
Sujet du journal:
MICROBIOLOGIA
Année:
2024
Type de document:
Article
Pays de publication:
Royaume-Uni