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Engineering a far-red fluorescent probe for rapid detection of Hg(II) ions in both cells and zebrafish.
Patthana, Patthavongsa; Zhong, Hai-Chen; Wu, Qian; Ren, Tian-Bing; Yuan, Lin.
Affiliation
  • Patthana P; State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
  • Zhong HC; State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
  • Wu Q; State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China. Electronic address: Qwu7@hnu.edu.cn.
  • Ren TB; State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
  • Yuan L; State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China. Electronic address: lyuan@hnu.edu.cn.
Spectrochim Acta A Mol Biomol Spectrosc ; 318: 124469, 2024 Oct 05.
Article de En | MEDLINE | ID: mdl-38776671
ABSTRACT
Abnormal accumulation of mercury ions (Hg2+) in organisms can lead to severe central nervous system and other diseases. Therefore, the monitoring and detection of Hg2+ are of great significance for human health and environmental safety. Herein, we designed and synthesized a novel far-red to NIR emission fluorescent probe (Rho-Hg) based on rhodamine derivative as the fluorophore and thiospirolactone as the recognition site for turn-on detecting of Hg2+ in living cells and zebrafish. The probe Rho-Hg displayed superior sensitivity (detection limit = 17.5 nM), rapid response (<1 min), colorimetric change, high selectivity, and moderate pH stability. Leveraging this probe, we realized the real-time monitoring of Hg2+ in real samples, living cells and zebrafish. By fostering zebrafish embryos and larvae in Hg2+-containing nutrient solution, we noticed that Hg2+ was ingested into the zebrafish liver when zebrafish were grown up to 3 days old, and thus we successfully monitored the accumulation and changes of Hg2+ during zebrafish growth and development. Thus, the probe Rho-Hg could be a powerful tool for sensitive and real-time monitoring of Hg2+ in living systems.
Sujet(s)

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Danio zébré / Colorants fluorescents / Mercure Limites: Animals / Humans Langue: En Journal: Spectrochim Acta A Mol Biomol Spectrosc Sujet du journal: BIOLOGIA MOLECULAR Année: 2024 Type de document: Article Pays d'affiliation: Chine Pays de publication: Royaume-Uni

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Danio zébré / Colorants fluorescents / Mercure Limites: Animals / Humans Langue: En Journal: Spectrochim Acta A Mol Biomol Spectrosc Sujet du journal: BIOLOGIA MOLECULAR Année: 2024 Type de document: Article Pays d'affiliation: Chine Pays de publication: Royaume-Uni