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Heat-activated growth of metastable and length-defined DNA fibers expands traditional polymer assembly.
Dore, Michael D; Rafique, Muhammad Ghufran; Yang, Tianxiao Peter; Zorman, Marlo; Platnich, Casey M; Xu, Pengfei; Trinh, Tuan; Rizzuto, Felix J; Cosa, Gonzalo; Li, Jianing; Guarné, Alba; Sleiman, Hanadi F.
Affiliation
  • Dore MD; Department of Chemistry, McGill University, 801 Sherbrooke St W, Montréal, QC, H3A 08B, Canada.
  • Rafique MG; Department of Chemistry, McGill University, 801 Sherbrooke St W, Montréal, QC, H3A 08B, Canada.
  • Yang TP; Department of Biochemistry and Centre de Recherche en Biologie Structurale, McGill University, Montréal, QC, Canada.
  • Zorman M; Department of Chemistry, University of Vermont, Burlington, VT, 05405, USA.
  • Platnich CM; Department of Chemistry, McGill University, 801 Sherbrooke St W, Montréal, QC, H3A 08B, Canada.
  • Xu P; Department of Chemistry, McGill University, 801 Sherbrooke St W, Montréal, QC, H3A 08B, Canada.
  • Trinh T; Department of Chemistry, McGill University, 801 Sherbrooke St W, Montréal, QC, H3A 08B, Canada.
  • Rizzuto FJ; School of Chemistry, University of New South Wales, Sydney, NSW, 2052, Australia.
  • Cosa G; Department of Chemistry, McGill University, 801 Sherbrooke St W, Montréal, QC, H3A 08B, Canada.
  • Li J; Centre de Recherche en Biologie Structurale, McGill University, Montréal, QC, Canada.
  • Guarné A; Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN, 47906, USA.
  • Sleiman HF; Department of Biochemistry and Centre de Recherche en Biologie Structurale, McGill University, Montréal, QC, Canada.
Nat Commun ; 15(1): 4384, 2024 May 23.
Article de En | MEDLINE | ID: mdl-38782917
ABSTRACT
Biopolymers such as nucleic acids and proteins exhibit dynamic backbone folding, wherein site-specific intramolecular interactions determine overall structure. Proteins then hierarchically assemble into supramolecular polymers such as microtubules, that are robust yet dynamic, constantly growing or shortening to adjust to cellular needs. The combination of dynamic, energy-driven folding and growth with structural stiffness and length control is difficult to achieve in synthetic polymer self-assembly. Here we show that highly charged, monodisperse DNA-oligomers assemble via seeded growth into length-controlled supramolecular fibers during heating; when the temperature is lowered, these metastable fibers slowly disassemble. Furthermore, the specific molecular structures of oligomers that promote fiber formation contradict the typical theory of block copolymer self-assembly. Efficient curling and packing of the oligomers - or 'curlamers' - determine morphology, rather than hydrophobic to hydrophilic ratio. Addition of a small molecule stabilises the DNA fibers, enabling temporal control of polymer lifetime and underscoring their potential use in nucleic-acid delivery, stimuli-responsive biomaterials, and soft robotics.
Sujet(s)

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Polymères / ADN / Température élevée Langue: En Journal: Nat Commun Sujet du journal: BIOLOGIA / CIENCIA Année: 2024 Type de document: Article Pays d'affiliation: Canada Pays de publication: Royaume-Uni

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Polymères / ADN / Température élevée Langue: En Journal: Nat Commun Sujet du journal: BIOLOGIA / CIENCIA Année: 2024 Type de document: Article Pays d'affiliation: Canada Pays de publication: Royaume-Uni