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The RNAi Machinery in the Fungus Fusarium fujikuroi Is Not Very Active in Synthetic Medium and Is Related to Transposable Elements.
Pardo-Medina, Javier; Dahlmann, Tim A; Nowrousian, Minou; Limón, M Carmen; Avalos, Javier.
Affiliation
  • Pardo-Medina J; Departamento de Genética, Facultad de Biología, Universidad de Sevilla, 41012 Seville, Spain.
  • Dahlmann TA; Department of Molecular and Cellular Botany, Ruhr-University Bochum, ND 7/176 Universitätsstr. 150, 44780 Bochum, Germany.
  • Nowrousian M; Department of Molecular and Cellular Botany, Ruhr-University Bochum, ND 7/176 Universitätsstr. 150, 44780 Bochum, Germany.
  • Limón MC; Departamento de Genética, Facultad de Biología, Universidad de Sevilla, 41012 Seville, Spain.
  • Avalos J; Departamento de Genética, Facultad de Biología, Universidad de Sevilla, 41012 Seville, Spain.
Noncoding RNA ; 10(3)2024 May 16.
Article de En | MEDLINE | ID: mdl-38804363
ABSTRACT
Small RNAS (sRNAs) participate in regulatory RNA interference (RNAi) mechanisms in a wide range of eukaryotic organisms, including fungi. The fungus Fusarium fujikuroi, a model for the study of secondary metabolism, contains a complete set of genes for RNAi pathways. We have analyzed by high-throughput sequencing the content of sRNAs in total RNA samples of F. fujikuroi grown in synthetic medium in the dark or after 1 h of illumination, using libraries below 150 nt, covering sRNAs and their precursors. For comparison, a parallel analysis with Fusarium oxysporum was carried out. The sRNA reads showed a higher proportion of 5' uracil in the RNA samples of the expected sizes in both species, indicating the occurrence of genuine sRNAs, and putative miRNA-like sRNAs (milRNAS) were identified with prediction software. F. fujikuroi carries at least one transcriptionally expressed Ty1/copia-like retrotransposable element, in which sRNAs were found in both sense and antisense DNA strands, while in F. oxysporum skippy-like elements also show sRNA formation. The finding of sRNA in these mobile elements indicates an active sRNA-based RNAi pathway. Targeted deletion of dcl2, the only F. fujikuroi Dicer gene with significant expression under the conditions tested, did not produce appreciable phenotypic or transcriptomic alterations.
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Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Langue: En Journal: Noncoding RNA Année: 2024 Type de document: Article Pays d'affiliation: Espagne Pays de publication: Suisse

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Langue: En Journal: Noncoding RNA Année: 2024 Type de document: Article Pays d'affiliation: Espagne Pays de publication: Suisse