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Distinct serum exosomal miRNA profiles detected in acute and asymptomatic dengue infections: A community-based study in Baiyun District, Guangzhou.
Li, Xiaokang; Liao, Conghui; Wu, Jiani; Yi, Boyang; Zha, Renyun; Deng, Qiang; Xu, Jianhua; Guo, Cheng; Lu, Jiahai.
Affiliation
  • Li X; School of Public Health, Sun Yat-Sen University, Guangzhou, 510080, China.
  • Liao C; One Health Center of Excellence for Research & Training, Sun Yat-Sen University, Guangzhou, 510080, China.
  • Wu J; School of Public Health, Sun Yat-Sen University, Guangzhou, 510080, China.
  • Yi B; One Health Center of Excellence for Research & Training, Sun Yat-Sen University, Guangzhou, 510080, China.
  • Zha R; School of Public Health, Sun Yat-Sen University, Guangzhou, 510080, China.
  • Deng Q; One Health Center of Excellence for Research & Training, Sun Yat-Sen University, Guangzhou, 510080, China.
  • Xu J; School of Public Health, Sun Yat-Sen University, Guangzhou, 510080, China.
  • Guo C; One Health Center of Excellence for Research & Training, Sun Yat-Sen University, Guangzhou, 510080, China.
  • Lu J; School of Public Health, Sun Yat-Sen University, Guangzhou, 510080, China.
Heliyon ; 10(10): e31546, 2024 May 30.
Article de En | MEDLINE | ID: mdl-38807894
ABSTRACT

Background:

In recent years, research on exosomal miRNAs has provided new insights into exploring the mechanism of viral infection and disease prevention. This study aimed to investigate the serum exosomal miRNA expression profile of dengue-infected individuals through a community survey of dengue virus (DENV) infection.

Methods:

A seroprevalence study of 1253 healthy persons was first conducted to ascertain the DENV infection status in Baiyun District, Guangzhou. A total of 18 serum samples, including 6 healthy controls (HC), 6 asymptomatic DENV infections (AsymptDI), and 6 confirmed dengue fever patients (AcuteDI), were collected for exosome isolation and then sRNA sequencing. Through bioinformatics analysis, we discovered distinct serum exosomal miRNA profiles among the different groups and identified differentially expressed miRNAs (DEMs). These findings were further validated by qRT-PCR.

Results:

The community survey of DENV infection indicated that the DENV IgG antibody positivity rate among the population was 11.97 % in the study area, with asymptomatic infected individuals accounting for 93.06 % of the anti-DENV IgG positives. The age and Guangzhou household registration were associated with DENV IgG antibody positivity by logistic regression analysis. Distinct miRNA profiles were observed between healthy individuals and DENV infections. A total of 1854 miRNAs were identified in 18 serum exosome samples from the initial analysis of the sequencing data. Comparative analysis revealed 23 DEMs comprising 5 upregulated and 18 downregulated miRNAs in the DENV-infected group (mergedDI). In comparison to AcuteDI, 18 upregulated miRNAs were identified in AsymptDI. Moreover, functional enrichment of the predicted target genes of DEMs indicated that these miRNAs were involved in biological processes and pathways related to cell adhesion, focal adhesion, endocytosis, and ECM-receptor interaction. Eight DEMs were validated by qRT-PCR.

Conclusion:

The Baiyun District of Guangzhou exhibits a notable proportion of asymptomatic DENV infections as suggested in other research, highlighting the need for enhanced monitoring and screening of asymptomatic persons and the elderly. Differential miRNA expression among healthy, symptomatic and asymptomatic DENV-infected individuals suggests their potential as biomarkers for distinguishing DENV infection and offers new avenues of investigating the mechanisms underlying DENV asymptomatic infections.
Mots clés

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Langue: En Journal: Heliyon Année: 2024 Type de document: Article Pays d'affiliation: Chine Pays de publication: Royaume-Uni

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Langue: En Journal: Heliyon Année: 2024 Type de document: Article Pays d'affiliation: Chine Pays de publication: Royaume-Uni