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GM1 Ameliorates Neuronal Injury in Rats after Cerebral Ischemia and Reperfusion: Potential Contribution of Effects on SPTBN1-mediated Signaling.
Shi, Yun-Wei; Xu, Chun-Cheng; Sun, Chun-Yan; Liu, Jia-Xing; Zhao, Shu-Yong; Liu, Dong; Fan, Xing-Juan; Wang, Cai-Ping.
Affiliation
  • Shi YW; Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Nantong University, Nantong 226001, Jiangsu, People's Republic of China; School of Life Scie
  • Xu CC; Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Nantong University, Nantong 226001, Jiangsu, People's Republic of China.
  • Sun CY; Qilu Pharmaceutical Co., Ltd., Ji'nan 250104, Shandong, People's Republic of China.
  • Liu JX; Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Nantong University, Nantong 226001, Jiangsu, People's Republic of China.
  • Zhao SY; Qilu Pharmaceutical Co., Ltd., Ji'nan 250104, Shandong, People's Republic of China.
  • Liu D; School of Life Science, Nantong Laboratory of Development and Diseases, Nantong University, Nantong 226019, Jiangsu, People's Republic of China. Electronic address: tom@ntu.edu.cn.
  • Fan XJ; Department of Neurology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, People's Republic of China. Electronic address: fxingjuan@163.com.
  • Wang CP; Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Nantong University, Nantong 226001, Jiangsu, People's Republic of China. Electronic address:
Neuroscience ; 551: 103-118, 2024 Jul 23.
Article de En | MEDLINE | ID: mdl-38810691
ABSTRACT
Monosialoganglioside GM1 (GM1) has long been used as a therapeutic agent for neurological diseases in the clinical treatment of ischemic stroke. However, the mechanism underlying the neuroprotective function of GM1 is still obscure until now. In this study, we investigated the effects of GM1 in ischemia and reperfusion (I/R) brain injury models. Middle cerebral artery occlusion and reperfusion (MCAO/R) rats were treated with GM1 (60 mg·kg-1·d-1, tail vein injection) for 2 weeks. The results showed that GM1 substantially attenuated the MCAO/R-induced neurological dysfunction and inhibited the inflammatory responses and cell apoptosis in ischemic parietal cortex. We further revealed that GM1 inhibited the activation of NFκB/MAPK signaling pathway induced by MCAO/R injury. To explore its underlying mechanism of the neuroprotective effect, transcriptome sequencing was introduced to screen the differentially expressed genes (DEGs). By function enrichment and PPI network analyses, Sptbn1 was identified as a node gene in the network regulated by GM1 treatment. In the MCAO/R model of rats and oxygen-glucose deprivation and reperfusion (OGD/R) model of primary culture of rat cortical neurons, we first found that SPTBN1 was involved in the attenuation of I/R induced neuronal injury after GM1 administration. In SPTBN1-knockdown SH-SY5Y cells, the treatment with GM1 (20 µM) significantly increased SPTBN1 level. Moreover, OGD/R decreased SPTBN1 level in SPTBN1-overexpressed SH-SY5Y cells. These results indicated that GM1 might achieve its potent neuroprotective effects by regulating inflammatory response, cell apoptosis, and cytomembrane and cytoskeleton signals through SPTBN1. Therefore, SPTBN1 may be a potential target for the treatment of ischemic stroke.
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Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Lésion d'ischémie-reperfusion / Transduction du signal / Rat Sprague-Dawley / Neuroprotecteurs / Ganglioside GM1 / Neurones Limites: Animals Langue: En Journal: Neuroscience Année: 2024 Type de document: Article

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Lésion d'ischémie-reperfusion / Transduction du signal / Rat Sprague-Dawley / Neuroprotecteurs / Ganglioside GM1 / Neurones Limites: Animals Langue: En Journal: Neuroscience Année: 2024 Type de document: Article