Construction of a novel aminofluorene-based ratiometric near-infrared fluorescence probe for detecting carboxylesterase activity in living cells.
Anal Methods
; 16(23): 3641-3645, 2024 Jun 13.
Article
de En
| MEDLINE
| ID: mdl-38812419
ABSTRACT
Herein, we constructed a novel aminofluorene-based fluorescence probe (FEN-CE) for the detection of carboxylesterase (CE) in living cells by a ratiometric near-infrared (NIR) fluorescence signal. FEN-CE with NIR emission (650 nm) could be hydrolyzed specifically by CE and transformed to FENH with the release of the self-immolative group, which exhibited a red-shifted emission peak of 680 nm. In addition, FEN-CE showed high selectivity for CE and was successfully used in the detection of CE activity in living cells through its ratiometric NIR fluorescence signals.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Carboxylesterase
/
Fluorènes
/
Colorants fluorescents
Limites:
Humans
Langue:
En
Journal:
Anal Methods
Année:
2024
Type de document:
Article
Pays de publication:
Royaume-Uni