Use of a head-to-tail peptide cyclase to prepare hybrid RiPPs.
Chem Commun (Camb)
; 60(51): 6508-6511, 2024 Jun 20.
Article
de En
| MEDLINE
| ID: mdl-38833296
ABSTRACT
Cyclotides and lanthipeptides are cyclic peptide natural products with promising bioengineering potential. No peptides have been isolated that contain both structural motifs defining these two families, an N-to-C cyclised backbone and lanthionine linkages. We combined their biosynthetic machineries to produce hybrid structures that possess improved activity or stability, demonstrate how the AEP-1 plant cyclase can be utilised to complete the maturation of the sactipeptide subtilosin A, and present head-to-tail cyclisation of the glycocin sublancin. These studies show the plasticity of AEP-1 and its utilisation alongside other post-translational modifications.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Cyclotides
Langue:
En
Journal:
Chem Commun (Camb)
Sujet du journal:
QUIMICA
Année:
2024
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique