Widely tunable fiber optical parametric oscillator synchronized with a Ti:sapphire laser for stimulated Raman scattering microscopy.
Biomed Opt Express
; 15(5): 3191-3199, 2024 May 01.
Article
de En
| MEDLINE
| ID: mdl-38855684
ABSTRACT
Stimulated Raman scattering (SRS) microscopy is a powerful vibrational imaging technique with high chemical specificity. However, the insufficient tuning range or speed of light sources limits the spectral range of SRS imaging and, hence, the ability to identify molecular species. Here, we present a widely tunable fiber optical parametric oscillator with a tuning range of 1470 cm-1, which can be synchronized with a Tisapphire laser. By using the synchronized light sources, we develop an SRS imaging system that covers the fingerprint and C-H stretching regions, without balanced detection. We validate its broadband imaging capability by visualizing a mixed polymer sample in multiple vibrational modes. We also demonstrate SRS imaging of HeLa cells, showing the applicability of our SRS microscope to biological samples.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Langue:
En
Journal:
Biomed Opt Express
Année:
2024
Type de document:
Article
Pays d'affiliation:
Japon