Kaempferol-Enhanced Migration and Differentiation of C2C12 Myoblasts via ITG1B/FAK/Paxillin and IGF1R/AKT/mTOR Signaling Pathways.
Mol Nutr Food Res
; 68(14): e2300685, 2024 Jul.
Article
de En
| MEDLINE
| ID: mdl-38860356
ABSTRACT
SCOPE Kaempferol (KMP), a bioactive flavonoid compound found in fruits and vegetables, contributes to human health in many ways but little is known about its relationship with muscle mass. The effect of KMP on C2C12 myoblast differentiation and the mechanisms that might underlie that effect are studied. METHODS AND RESULTS:
This study finds that KMP (1, 10 µM) increases the migration and differentiation of C2C12 myoblasts in vitro. Studying the possible mechanism underlying its effect on migration, the study finds that KMP activates Integrin Subunit Beta 1 (ITGB1) in C2C12 myoblasts, increasing p-FAK (Tyr398) and its downstream cell division cycle 42 (CDC42), a protein previously associated with cell migration. Regarding differentiation, KMP upregulates the expression of myosin heavy chain (MHC) and activates IGF1/AKT/mTOR/P70S6K. Interestingly, pretreatment with an AKT inhibitor (LY294002) and siRNA knockdown of IGF1R leads to a decrease in cell differentiation, suggesting that IGF1/AKT activation is required for KMP to induce C2C12 myoblast differentiation.CONCLUSION:
Together, the findings suggest that KMP enhances the migration and differentiation of C2C12 myoblasts through the ITG1B/FAK/paxillin and IGF1R/AKT/mTOR pathways. Thus, KMP supplementation might potentially be used to prevent or delay age-related loss of muscle mass and help maintain muscle health.Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Transduction du signal
/
Différenciation cellulaire
/
Mouvement cellulaire
/
Récepteur IGF de type 1
/
Antigènes CD29
/
Myoblastes
/
Kaempférols
/
Protéines proto-oncogènes c-akt
/
Paxilline
/
Sérine-thréonine kinases TOR
Limites:
Animals
Langue:
En
Journal:
Mol Nutr Food Res
Sujet du journal:
CIENCIAS DA NUTRICAO
Année:
2024
Type de document:
Article
Pays d'affiliation:
Taïwan